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3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

4D Multimodality Imaging of Citrobacter rodentium Infections in Mice

James William Collins¹, Jeffrey A Meganck², Chaincy Kuo², Kevin P Francis², Gad Frankel¹

¹MRC Centre for Molecular Bacteriology and Infection, Division of Cell & Molecular Biology, Imperial College London, ²Preclinical Imaging, Caliper- A PerkinElmer Company

Multi-modality imaging is a valuable approach for studying bacterial colonization in small animal models. This protocol outlines infection of mice with bioluminescent Citrobacter rodentium and the longitudinal monitoring of bacterial colonization using composite 3D diffuse light imaging tomography with μCT imaging to create a 4D movie of C. rodentium infection.

Medicine

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

Xueli Zhang^1,2, Chaincy Kuo³, Anna Moore¹, Chongzhao Ran¹

¹Molecular Imaging Laboratory, MGH/MIT/HMS Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital/Harvard Medical School, ²Center for Drug Discovery, School of Pharmacy, China Pharmaceutical University, ³Perkin Elmer

In this video report, we show the application of Cerenkov Luminescence Imaging (CLI) for interscapular brown adipose tissue in mice under activated and depressed conditions.

Cancer Research

Multimodal Bioluminescent and Positronic-emission Tomography/Computational Tomography Imaging of Multiple Myeloma Bone Marrow Xenografts in NOG Mice

Gilbert Gastelum¹, Eric Y. Chang², David Shackleford³, Nicholas Bernthal³, Jeffery Kraut^1,3, Kevin Francis⁴, Victoria Smutko¹, Patrick Frost^1,3

¹Greater Los Angeles Veteran Administration Healthcare System, ²San Diego Veterans Administration Healthcare System, ³University of California, Los Angeles, ⁴Perkin Elmer

Here we use bioluminescent, X-ray, and positron-emission tomography/computed tomography imaging to study how inhibiting mTOR activity impacts bone marrow-engrafted myeloma tumors in a xenograft model. This allows for physiologically relevant, non-invasive, and multimodal analyses of the anti-myeloma effect of therapies targeting bone marrow-engrafted myeloma tumors in vivo.