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National Taiwan University College of Medicine

4 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System

Shiun Chang¹, Li-Mei Pai^2,3, Chien-Kuo Lee¹

¹Graduate Institute of Immunology, National Taiwan University College of Medicine, ²Department of Biochemistry, Chang Gung University College of Medicine, ³Molecular Medicine Research Center, Chang Gung University College of Medicine

In this study we present an in vitro culture system that can efficiently generate pDCs by co-culturing common lymphoid progenitors with AC-6 feeder cells in the presence of Flt3 ligand.

Immunology and Infection

Development of an Economical DNA Delivery System by "Acufection" and its Application to Skin Research

Yu-Jei Lin¹, Tsung-Lin Lee¹, Chia-Chi Ku¹

¹Graduate Institute of Immunology, National Taiwan University College of Medicine

This protocol is a cost-effective alternative for expressing naked plasmid DNA in mouse skin. The overall goal of the protocol is to deliver immune-related genes into skin tissue to delineate the functional role of a specific gene in cutaneous inflammation.

Immunology and Infection

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Yu-Ling Hsiao¹, Hans Häcker², Chien-Kuo Lee¹

¹Graduate Institute of Immunology, National Taiwan University College of Medicine, ²Department of Pathology, Division of Microbiology and Immunology, University of Utah

Here we provide a protocol for screening potential transcription factors involved in the development of dendritic cell (DC) using lentiviral transduction of shRNA to obtain stable knockdown cell lines for in vitro DC differentiation.

Immunology and Infection

A Clinically Relevant Murine Model of Peritoneal Fibrosis by Dialysate and Catheters

Yi-Ting Chen^1,2,3, Shuei-Liong Lin^1,2,3

¹Department of Integrated Diagnostics & Therapeutics, National Taiwan University Hospital, ²Renal Division, Department of Internal Medicine, National Taiwan University Hospital, ³Graduate Institute of Physiology, National Taiwan University College of Medicine

Patients with severe peritoneal fibrosis have high morbidity and mortality. The mechanism of peritoneal fibrosis is unclear. In this study, we describe a simple experimental murine model of peritoneal fibrosis induced by the injection of peritoneal dialysis fluid.