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University of Groningen, University Medical Center Groningen

3 ARTICLES PUBLISHED IN JoVE

Medicine

Ex Situ Normothermic Machine Perfusion of Donor Livers

Negin Karimian^1,2, Alix P.M. Matton^1,2, Andrie C. Westerkamp^1,2, Laura C. Burlage^1,2, Sanna op den Dries^1,2, Henri G.D. Leuvenink², Ton Lisman^1,2, Korkut Uygun³, James F. Markmann⁴, Robert J. Porte^1,2

¹Section of Hepato-Pancreato-Biliary Surgery and Liver Transplantation, University of Groningen, University Medical Center Groningen, ²Surgical Research Laboratory, Department of Surgery, University of Groningen, University Medical Center Groningen, ³Center of Engineering in Medicine/Surgical Services, Massachusetts General Hospital, Harvard Medical School, and Shriners Burns Hospital, ⁴Division of Transplantation, Department of Surgery, Massachusetts General Hospital, Harvard Medical School

Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.

Medicine

Functional Human Liver Preservation and Recovery by Means of Subnormothermic Machine Perfusion

Bote G. Bruinsma^*1, James H. Avruch^*2, Pepijn D. Weeder¹, Gautham V. Sridharan¹, Basak E. Uygun¹, Negin G. Karimian¹, Robert J. Porte³, James F. Markmann², Heidi Yeh², Korkut Uygun¹

¹Center for Engineering in Medicine, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School, ²Transplant Center, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School, ³Section of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, University Medical Center Groningen, University of Groningen

We describe a method of ex vivo machine perfusion of human liver grafts at subnormothermic temperature (21 °C).

JoVE Journal

Induction and Validation of Cellular Senescence in Primary Human Cells

Alejandra Hernandez-Segura¹, Simone Brandenburg¹, Marco Demaria¹

¹European Research Institute for the Biology of Aging, University of Groningen, University Medical Center Groningen

Here, we discuss a series of protocols for induction and validation of cellular senescence in cultured cells. We focus on different senescence-inducing stimuli and describe the quantification of common senescence-associated markers. We provide technical details using fibroblasts as a model, but the protocols can be adapted to various cellular models.