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Dnepropetrovsk State University

4 ARTICLES PUBLISHED IN JoVE

Neuroscience

Laser-Induced Brain Injury in the Motor Cortex of Rats

Ruslan Kuts^*1, Israel Melamed^*2, Honore N. Shiyntum³, Benjamin F. Gruenbaum⁴, Dmitry Frank¹, Boris Knyazer⁵, Dmitry Natanel¹, Olena Severynovska³, Max Vinokur¹, Matthew Boyko¹

¹Division of Anesthesiology and Critical Care, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, ²Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, ³Department of Biochemistry and Physiology, Faculty of Biology, Ecology, and Medicine, Oles Honchar Dnipro National University, ⁴Department of Anesthesiology, Yale University School of Medicine, ⁵Department of Ophthalmology, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev

The protocol presented here shows a technique to create a rodent model of brain injury. The method described here uses laser irradiation and targets motor cortex.

Neuroscience

Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples

Dmitry Frank^*1, Benjamin F. Gruenbaum^*2, Julia Grinshpun¹, Israel Melamed³, Olena Severynovska⁴, Ruslan Kuts¹, Michael Semyonov¹, Evgeni Brotfain¹, Alexander Zlotnik¹, Matthew Boyko¹

¹Department of Anesthesiology and Critical Care, Soroka Medical Center, Ben-Gurion University of the Negev, ²Department of Anesthesiology, Yale University School of Medicine, ³Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, ⁴Department of Physiology, Faculty of Biology, Ecology and Medicine, Dnepropetrovsk State University

This protocol describes a novel technique of measuring the three most important parameters of ischemic brain injury on the same set of rodent brain samples. Using only one brain sample is highly advantageous in terms of ethical and economic costs.

Neuroscience

A Metric Test for Assessing Spatial Working Memory in Adult Rats Following Traumatic Brain Injury

Dmitry Frank^*1, Benjamin F. Gruenbaum^*2, Israel Melamed³, Julia Grinshpun¹, Yair Benjamin¹, Ievgeni Vzhetson¹, Nadia Kravchenko⁴, Michael Dubilet¹, Matthew Boyko^*1, Alexander Zlotnik^*1

¹Department of Anesthesiology and Critical Care, Soroka Medical Center, Ben-Gurion University of the Negev, ²Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, ³Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, ⁴Department of Physiology, Faculty of Biology, Ecology and Medicine, Dnepropetrovsk State University

Traumatic brain injury (TBI) is commonly associated with memory impairment. Here, we present a protocol to assess spatial working memory after TBI via a metric task. A metric test is a useful tool to study spatial working memory impairment after TBI.

Behavior

Assessing Dominant-Submissive Behavior in Adult Rats Following Traumatic Brain Injury

Dmitry Frank^*1, Benjamin F. Gruenbaum^*2, Michael Semyonov¹, Yair Binyamin¹, Olena Severynovska³, Ron Gal¹, Amit Frenkel¹, Boris Knazer⁴, Matthew Boyko¹, Alexander Zlotnik¹

¹Division of Anesthesiology and Critical Care, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, ²Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, ³Department of Biochemistry and Physiology of the Faculty of Biology and Ecology, Oles Gonchar of the Dnipro National University, ⁴Department of Ophthalmology, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev

The present protocol describes a rat model of fluid percussion-induced traumatic brain injury followed by a series of behavioral tests to understand the development of dominant and submissive behavior. Using this model of traumatic brain injury in conjunction with specific behavioral tests enables the study of social impairments following brain injury.