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Zhejiang Cancer Hospital

3 ARTICLES PUBLISHED IN JoVE

Medicine

Establishment of a Simple and Effective Rat Model for Intraoperative Parathyroid Gland Imaging

Fan Chen^*1,2, Chun Liu^*3, Peng Guo³, Weihui Zheng^1,2

¹Key Laboratory of Head & Neck Cancer Translational Research of Zhejiang Province, Zhejiang Cancer Hospital, ²The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), ³Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences

To date, the development of parathyroid gland (PG) identification methods is limited by the lack of animal models in preclinical research. Here, we establish a simple and effective rat model for intraoperative PG imaging and evaluate its effectiveness by using iron oxide nanoparticles as a novel PG contrast agent.

Biology

A Magnetic Separation-Assisted High-Speed Homogenization Method for Large-Scale Production of Endosome-Derived Vesicles

Dujuan Wang^1,2, Shili Yao^2,3, Peng Guo^1,2,3,4

¹Academy of Medical Engineering and Translational Medicine, Tianjin University, ²Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, ³School of Materials Science and Engineering, Tianjin University, ⁴Zhejiang Cancer Hospital

Here, we describe a magnetic separation-assisted high-speed homogenization method for large-scale production of endosome-derived nanovesicles as a new type of exosome mimics (EMs) that share the same biological origin and similar structure, morphology, and protein composition of native extracellular vesicles (EVs).

Immunology and Infection

Assessing Antibody-dependent, Cell-mediated Cytotoxicity in Cancer Cells using Antibody-Dependent Cell-Mediated Cytotoxicity Reporter Bioassay

Kelvin Kam Yew Kuan^*1, Ke Zhang^*1,2, Koon Hwee Ang¹, Min Thura¹, Wei Hui Zheng^3,4

¹Institute of Molecular and Cell Biology (IMCB), A*STAR 61 Biopolis Drive, ²Department of Radiation Oncology, Affiliated Hangzhou Cancer Hospital, ³Key Laboratory of Head and Neck Cancer Translational Research of Zhejiang Province, Zhejiang Cancer Hospital, ⁴Department of Thyroid Surgery of Zhejiang Cancer Hospital

Here, we present a protocol for an antibody-dependent, cell-mediated cytotoxicity (ADCC) assay using an ADCC bioassay kit. This method offers a valuable tool for elucidating the ADCC mechanism and evaluating the therapeutic potential of antibodies in cancer immunotherapy.