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Rutgers University

4 ARTICLES PUBLISHED IN JoVE

Biology

Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters

Nada N. Boustany¹, Robert M. Pasternack¹, Jing-Yi Zheng¹

¹Department of Biomedical Engineering, Rutgers University

We demonstrate a dark-field microscopy method based on Gabor-like filtering to measure subcellular dynamics within single living cells. The technique is sensitive to alterations in the structure of organelles, such as mitochondrial fragmentation.

Biology

Herbivore-induced Blueberry Volatiles and Intra-plant Signaling

Cesar R. Rodriguez-Saona¹

¹Department of Entomology, Rutgers University

A push-pull method for collecting plant volatiles is described. The method allows for a comparison of volatiles induced by herbivore feeding, exogenous methyl jasmonate, and mechanical damage. This technique is also used to investigate the volatile response of undamaged branches to exposure to volatiles from herbivore-damaged branches within blueberry plants.

Biology

In Ovo Electroporation in Embryonic Chick Retina

Mohammed M. Islam¹, Sung Tae Doh², Li Cai^1,2

¹Department of Pharmacology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, ²Department of Biomedical Engineering, Rutgers University

The overall goal of this video is to show how to perform targeted retinal injection and in ovo electroporation of DNA/RNA constructs into the chick embryonic retina at the Hamburger and Hamilton stage 22-23, which is about embryonic day 4 (E4). This technique is very useful to study gene expression, gene regulation, and morphological change in developing chick retina.

Biology

Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins

Andrius Masedunskas^1,2, Natalie Porat-Shliom¹, Muhibullah Tora¹, Oleg Milberg^1,3, Roberto Weigert¹

¹Intracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch National Institute of Dental and Craniofacial Research, National Institutes of Health, ²Department of Biology, University of North Carolina at Chapel Hill , ³Department of Chemical & Biochemical Engineering and Department of Biomedical Engineering, Rutgers University

Intravital microscopy is a powerful tool that enables imaging various biological processes in live animals. In this article, we present a detailed method for imaging the dynamics of subcellular structures, such as the secretory granules, in the salivary glands of live mice.