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The University of Western Australia

5 ARTICLES PUBLISHED IN JoVE

Biology

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

Marcus K. Giacci¹, Nathan S. Hart², Richard V. Hartz¹, Alan R. Harvey³, Stuart I. Hodgetts³, Melinda Fitzgerald¹

¹Experimental and Regenerative Neurosciences, School of Animal Biology, The University of Western Australia, ²School of Animal Biology and The Oceans Institute, The University of Western Australia, ³Experimental and Regenerative Neurosciences, School of Anatomy, Physiology and Human Biology, The University of Western Australia

Non-coherent Xenon light was passed through narrow-band interference and neutral density filters to deliver light of varying wavelength and intensity to cultured cells. This protocol was used to assess the effects of red/near-infrared light therapy on production of reactive species in vitro: no effects were observed using the tested parameters.

Immunology and Infection

A Modified Murine Heterotopic Heart Transplant Protocol Matching Contemporary Standards of Aseptic Technique, Anesthesia, and Analgesia

David A. Singer¹, Gabrielle C. Musk², Wen Hua Huang^1,3, Liu Liu¹, Jaskirat Kaur¹, Monalyssa Watson¹, Amy Prosser¹, Michaela Lucas^1,4, Andrew Lucas⁵

¹Medical School, The University of Western Australia, ²Animal Care Services, The University of Western Australia, ³Western Australian Liver and Kidney Transplant Service, Sir Charles Gairdner Hospital, ⁴Department of Immunology, Sir Charles Gairdner Hospital and Pathwest Laboratory Medicine, ⁵School of Biomedical Sciences, The University of Western Australia

The present paper describes a modified technique for heterotopic vascularized cardiac transplantation with updated aseptic technique, analgesia, and anesthesia.

Biology

Fluorescence Lifetime Macro Imager for Biomedical Applications

Rajannya Sen¹, Alexander V. Zhdanov¹, Ciaran Devoy², Mark Tangney², Liisa M. Hirvonen³, Andrei Nomerotski⁴, Dmitri B. Papkovsky¹

¹School of Biochemistry and Cell Biology, University College Cork, ²Cancer Research@UCC, University College Cork, ³Centre for Microscopy, Characterisation and Analysis (CMCA), The University of Western Australia, ⁴Physics Department, Brookhaven National Laboratory

This paper describes the use of a new, fast optical imager for the macroscopic photoluminescence lifetime imaging of long decay emitting samples. The integration, image acquisition, and analysis procedures are described, along with the preparation and characterization of the sensor materials for the imaging and the application of the imager in studying biological samples.

Medicine

Continuous Telemetric In Utero Tracheal Pressure Measurements in Fetal Lambs

Mar Janna Dahl¹, Stefano Robbiani², Chiara Veneroni², Anna Lavizzari³, Sebastian Amos¹, Gabrielle C. Musk^1,4, Helen Kershaw⁴, Jonathan W. Davis^5,6, Fabio Mosca³, Peter B. Noble¹, Raffaele Dellacà^*2, J. Jane Pillow^*1,6

¹School of Human Sciences, The University of Western Australia, ²Dipartimento di Elettronica, Informazione e Bioingegneria, Politecnico di Milano University, ³NICU, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, ⁴Animal Care Services, The University of Western Australia, ⁵Medical School, University of Western Australia, ⁶Telethon Kids Institute

Optimization of occlusion pressure and duration required to improve lung hypoplasia with tracheal occlusion is essential to improve in utero corrective treatments for congenital diaphragmatic hernia (CDH). This study reports a new method for continuous measurement of the tracheal pressure in an occluded and unoccluded fetal lamb surgical model of CDH.

Immunology and Infection

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance

Liang Wang^*1,2,3, Jin-Xin Lai^*1, Yu-Ting Si^*1,4, Xu-Xia Cui^1,4, Zeeshan Umar^1,5, Xiao-Jun Ru¹, Xin-Yu Zhang¹, Zheng-Kang Li¹, Alfred Chin Yen Tay^5,6,7,8, Barry J. Marshall^5,6,7,8, Guang-Hua Li¹, Bing Gu¹

¹Laboratory Medicine, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, ²Division of Microbiology and Immunology, School of Biomedical Sciences, The University of Western Australia, ³Center for Precision Health, School of Medical and Health Sciences, Edith Cowan University, ⁴Medical Technology School of Xuzhou Medical University, ⁵Marshall Laboratory of Biomedical Engineering, School of Medicine, Shenzhen University, ⁶The Marshall Centre for Infectious Diseases Research and Training, The University of Western Australia, ⁷Marshall International Digestive Diseases Hospital, Zhengzhou University, ⁸Marshall Medical Research Center, Fifth Affiliated Hospital of Zhengzhou University

The protocol presents a noninvasive method for the rapid diagnosis of Helicobacter pylori stomach infections through the string test and determines its antibiotic resistance to clarithromycin and levofloxacin using quantitative polymerase chain reaction (qPCR).