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Mackay Memorial Hospital

5 ARTICLES PUBLISHED IN JoVE

Medicine

A Model of Cardiac Remodeling Through Constriction of the Abdominal Aorta in Rats

Hui-Chun Ku¹, Shih-Yi Lee^2,3, Yuan-Kun Aden Wu⁴, Kai-Chien Yang^1,5, Ming-Jai Su¹

¹Graduate Institute of Pharmacology, College of Medicine, National Taiwan University, ²Division of Pulmonary and Critical Care Medicine, Mackay Memorial Hospital, ³Mackay Medicine, Nursing and Management College, ⁴School of Medicine, National Taiwan University, ⁵Department of Internal Medicine, National Taiwan University Hosptial

A rat model of abdominal aortic constriction that induces cardiac hypertrophy and remodeling is described. An efficient, highly-reproducible, and minimally-invasive method is used to provide a simple yet useful platform for research in myocardial hypertrophy and dysfunction.

Medicine

An Endoscopic Transcanal Transpromontorial Approach for Vestibular Schwannomas using a Computer-Based Three-Dimensional Imaging System

Chin-Kuo Chen¹, Li-Chun Hsieh^2,3, Yu-Jen Lu⁴, Chu-Po Lin⁵

¹Department of Otolaryngology-Head and Neck Surgery and Communication Enhancement Center, Chang Gung Memorial Hospital and Chang Gung University, ²Department of Otolaryngology-Head and Neck Surgery, Mackay Memorial Hospital, ³Department of Audiology and Speech Language Pathology, Mackay Medical College, ⁴Department of Neurosurgery, Chang Gung Memorial Hospital and Chang Gung University, ⁵Department of Medical Education, Chang Gung Memorial Hospital and Chang Gung University

Here, we present a transcanal transpromontorial approach for vestibular schwannomas using a computer-based three-dimensional (3D) imaging system combined with a two-dimensional (2D) endoscope. This system provided stereoscopic vision, better depth perception and reduced visual fatigue. This 3D imaging system enabled the application of 3D vision technology in endoscopic lateral skull base surgery.

Cancer Research

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Chun-Chia Cheng¹, Po-Jui Hsu⁴, Zong-Lin Sie¹, Fang-Hsin Chen^1,2,3

¹Radiation Biology Research Center, Institute for Radiological Research, Chang Gung University, Chang Gung Memorial Hospital at Linkou, ²Department of Medical Imaging and Radiological Sciences, Chang Gung University, ³Department of Radiation Oncology, Chang Gung Memorial Hospital, ⁴Department of Laboratory Medicine, Mackay Memorial Hospital

Presented here is a protocol to discover the overexpressed driver genes maintaining the established cancer stem-like cells derived from colorectal HT29 cells. RNAseq with available bioinformatics was performed to investigate and screen gene expression networks for elucidating a potential mechanism involved in the survival of targeted tumor cells.

Medicine

Left Anterior Descending Coronary Artery Ligation for Ischemia-Reperfusion Research: Model Improvement via Technical Modifications and Quality Control

Hui-Chun Ku¹, Ding-Kuo Chien^2,3,4,5, Chuan-Lei Chao^5,6, Shih-Yi Lee^5,7

¹Department of Life Science, Fu Jen Catholic University, ²Department of Medicine, MacKay Medical College, ³Department of Emergency Medicine, MacKay Memorial Hospital, ⁴Graduate Institute of Injury Prevention and Control, Taipei Medical University, ⁵MacKay Junior College of Medicine, Nursing, and Management, ⁶Division of Cardiology, Department of Internal Medicine, Taitung Mackay Memorial Hospital, ⁷Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, MacKay Memorial Hospital

Herein, we present a protocol focusing on the quality control of the left anterior descending coronary artery ligation by technically modifying the traditional procedure in rats for acute myocardial ischemia-reperfusion research.

Medicine

Technical Refinement of a Bilateral Renal Ischemia-Reperfusion Mouse Model for Acute Kidney Injury Research

Hui-Chun Ku¹, Cheng-Wei Huang¹, Shih Yi Lee^2,3

¹Department of Life Science, Fu Jen Catholic University, ²MacKay Junior College of Medicine, Nursing, and Management, ³Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, MacKay Memorial Hospital

This study established a protocol focusing on the technical refinement of a mouse model of bilateral renal ischemia-reperfusion for acute kidney injury research.