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University California San Diego

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

A Syngeneic Pancreatic Cancer Mouse Model to Study the Effects of Irreversible Electroporation

Jayanth S. Shankara Narayanan¹, Partha Ray¹, Ibtehaj Naqvi², Rebekah White¹

¹Moores Cancer Center, University California San Diego, ²Duke University School of Medicine

Irreversible electroporation (IRE) is a non-thermal ablation technique used for the treatment of locally advanced pancreatic cancer. Being a relatively new technique, the effects of IRE on the tumor growth are poorly understood. We have developed a syngeneic mouse model that facilitates studying the effects of IRE on pancreatic cancer.

Genetics

Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas

Francesca Boscolo Sesillo^1,2,3, Alessandra Sacco²

¹Graduate School of Biomedical Sciences, Sanford Burnham Prebys Medical Discovery Institute, ²Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, ³Department of Obstetrics, Gynecology, and Reproductive Sciences, Division of Female Pelvic Medicine and Reconstructive Surgery, University California San Diego

This protocol describes a reproducible method for isolation of mouse rhabdomyosarcoma primary cells, tumorsphere formation and treatment, and allograft transplantation starting from tumorspheres cultures.