University California San Diego
2 ARTICLES PUBLISHED IN JoVE
Cancer Research
A Syngeneic Pancreatic Cancer Mouse Model to Study the Effects of Irreversible Electroporation
Jayanth S. Shankara Narayanan 1, Partha Ray 1, Ibtehaj Naqvi 2, Rebekah White 1
1Moores Cancer Center, University California San Diego, 2Duke University School of Medicine
Irreversible electroporation (IRE) is a non-thermal ablation technique used for the treatment of locally advanced pancreatic cancer. Being a relatively new technique, the effects of IRE on the tumor growth are poorly understood. We have developed a syngeneic mouse model that facilitates studying the effects of IRE on pancreatic cancer.
Genetics
Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas
Francesca Boscolo Sesillo 1,2,3, Alessandra Sacco 2
1Graduate School of Biomedical Sciences, Sanford Burnham Prebys Medical Discovery Institute, 2Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, 3Department of Obstetrics, Gynecology, and Reproductive Sciences, Division of Female Pelvic Medicine and Reconstructive Surgery, University California San Diego
This protocol describes a reproducible method for isolation of mouse rhabdomyosarcoma primary cells, tumorsphere formation and treatment, and allograft transplantation starting from tumorspheres cultures.
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