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Universidad Nacional de Rosario (UNR)

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase

Victoria Lucia Alonso^1,2, Romina Manarin¹, Virginia Perdomo¹, Ernesto Gulin³, Esteban Serra^1,2, Pamela Cribb^1,2

¹Área Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), ²Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, ³Instituto de Investigaciones Biomédicas (INBIOMED), Universidad de Buenos Aires (UBA) Facultad de Medicina, CONICET

We describe a high-throughput colorimetric assay measuring β-galactosidase activity in three life cycle stages of Trypanosoma cruzi, the causative agent of Chagas disease. This assay can be used to identify trypanocidal compounds in an easy, fast, and reproducible manner.

Biology

Ultrastructural Expansion Microscopy in Three In Vitro Life Cycle Stages of Trypanosoma cruzi

María Azul de Hernández^*1, Gonzalo Martinez Peralta^*1,2, Rodrigo Vena^1,4, Victoria Lucia Alonso^1,3,4

¹Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, ²Area Biología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), ³Area Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), ⁴Unidad de Microscopía, Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET

This study shows a detailed protocol to perform ultrastructure expansion microscopy in three in vitro life cycle stages of Trypanosoma cruzi, the pathogen responsible for Chagas disease. We include the optimized technique for cytoskeletal proteins and pan-proteome labeling.