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University of Oklahoma Health Sciences Center and Center for Pregnancy and Newborn Health

2 ARTICLES PUBLISHED IN JoVE

Medicine

Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

Adam Wilson^*1, Kathryn Burge^*1, Jeffrey Eckert¹, Hala Chaaban¹

¹Department of Pediatrics, Division of Neonatal-Perinatal Medicine, University of Oklahoma Health Sciences Center

This protocol describes a method to establish and perform a scratch wound assay on two-dimensional (2D) monolayers derived from three-dimensional (3D) enteroids isolated from non-human primate ileum.

Medicine

In Vitro Apical-Out Enteroid Model of Necrotizing Enterocolitis

Kathryn Burge¹, Adam Wilson¹, Hala Chaaban¹

¹Department of Pediatrics, Division of Neonatal-Perinatal Medicine, University of Oklahoma Health Sciences Center and Center for Pregnancy and Newborn Health

This protocol describes an apical-out necrotizing enterocolitis (NEC)-in-a-dish model utilizing small intestinal enteroids with reversed polarity, allowing access to the apical surface. We provide an immunofluorescent staining protocol to detect NEC-related epithelial disruption and a method to determine the viability of apical-out enteroids subjected to the NEC-in-a-dish protocol.