<ol style="padding:5px;"><li>セットアップ機器<ol style="padding:5px;"><li>室温が20℃以上であるラボのエリア℃を選択します。 </li><li>エアコンの通気口および/または大きな音近くは避けてください。 </li><li>そのような香水、オーデコロン、化学薬品、および洗剤のように、刺激および/またはストレスの原因となる臭いに動物を放置しないでください。 </li><li> CODAのコントローラをオンにします。 </li><li>温暖化プラットフォームをオンにして、レベル3に設定します。室温が25℃を超える場合は、レベル1または2で開始します。 </li><li>コントローラ診断テスト<ol style="padding:5px;"><li> CODAのソフトウェアを開きます。 </li><li>それをクリックして、CODAのデバイスを選択してください。 </li><li> &quot;テストの選択したデバイス&quot;をクリックしてください。 </li><li> &quot;Test&quot;をテストし、クリックして袖口とチャンネルを選択します。 </li><li>テストが完了したら、デバイステストウィンドウを閉じてください。 </li></ol></li></ol></li><li>セットアップソフトウェア<ol style="padding:5px;"><li>人事や動物を管理する<ol style="padding:5px;"><li>人事を管理する<ol style="padding:5px;"><li> [ツール]&gt;人事管理。 </li><li>名前を変更する&quot;研究1&quot;をクリックしてください。 </li><li>追加の研究者を追加するには、後続の行をクリックしてください。 </li><li>技術者を変更または追加するには、&quot;技術者のタブ&quot;をクリックしてください。 </li><li>ラットやマウス、識別しやすいように動物の名前を指定すると、動物の種類を選択するには&quot;標本のタブ&quot;をクリックしてください。 </li><li>動物の種類や感度を変更する&quot;動物のタブ&quot;をクリックしてください。 </li></ol></li></ol></li><li>新しい実験<ol style="padding:5px;"><li> [ファイル]&gt; [新規作成]&gt; [実験]を選択します</li><li>新しい実験を始める<ol style="padding:5px;"><li>実験名を入力してください。 </li><li>キー研究を選択してください。 </li><li>開始日を選択し、&quot;Next&quot;をクリックします。 </li></ol></li><li>基本的なセッション情報<ol style="padding:5px;"><li>セッション名を入力します。 </li><li> 5に&quot;馴化のサイクル&quot;を設定します。あなたがゼロに設定順化サイクルを、インクルードしたくない場合。ホルダーと袖口に動物を順化を支援するために、あなたの実験を追加サイクルを追加し、5日間連続の実験を実行する。 </li><li> &quot;セットの数&quot;を1に設定します。 </li><li>複数のセットを30秒に、&quot;セット間の時間&quot;を設定</li><li> &quot;セット当たりのサイクル数&quot;20サイクルに設定します。 </li><li> 5秒に&quot;サイクルの間の時間&quot;を設定し、&quot;次へ&quot;をクリックします。 </li></ol></li></ol></li><li>標本の選択<ol style="padding:5px;"><li style="list-style-type:none;"><ol style="padding:5px;"><li>試料のプールから標本を選択して、最初に使用可能なチャネルに割り当て、&quot;次へ&quot;をクリックします。 </li></ol></li><li>セッションパラメータ<ol style="padding:5px;"><li> 250mmHgに&quot;最大閉塞圧を&quot;設定してください。 </li><li>マウス、ラットの15秒20秒に&quot;デフレの時間&quot;を設定します。 </li><li> 15ulの&quot;最小音量&quot;を設定します。 </li><li>グラフごとに1つのチャンネルに&quot;表示スタイル&quot;を設定します。 </li><li>追加の研究者や技術者を追加し、&quot;次へ&quot;をクリックします。 </li></ol></li><li>セッションスクリプト<ol style="padding:5px;"><li>セッションスクリプトを確認し、&quot;次へ&quot;をクリックします。 </li><li>あなたが動物の準備のセクションを完了するまで、[完了]を選択しないでください。 </li></ol></li></ol></li></ol></li><li>動物の準備<ol style="padding:5px;"><li>動物のセットアップ<ol style="padding:5px;"><li>アニマルホルダー<ol style="padding:5px;"><li>訓練と動物の順化を向上させる、あなたの実験を追加サイクルを追加し、5日連続で実験を実行する。また、実際の調査に先立って3日間連続で15分間保持者に動物を置くことにより順化を支援することができます。 </li><li>尾で動物を拾って、ゆっくりとノーズコーンの開放端に直面しているホルダーの後部に動物を置くことによって、ホルダーにそれぞれの動物を置きます。注：選択したホルダーのサイズとの関係で動物の大きさ。動物は自由にホルダーを入力することができるはずです。 </li><li>慎重に後部のハッチに赤いスクリューキャップを回してホルダーに後部のハッチを固定します。ケアは、後部のハッチを確保しつつ、尾やその他の体の部分を挟まないように注意する必要があります。 </li><li>動物の動きを制限し、後部のハッチに向かってノーズコーンをスライドさせます。ノーズコーンは、ホルダーの内側であるときに振り返ってから動物を制限するために位置にあるはずです。 </li><li>指定された位置での温暖化のプラットフォームの上にホルダーを置きます。ある期間にわたって、動物の同じグループをテストする場合、あなたのBP測定値の一貫性と精度を向上させるために地球温暖化のプラットフォーム上でホルダーの位置を回転するようにしてください。少なくとも5分は、ホルダーに順応するために動物を許可する。 </li><li>ホルダー内部の間に動物に触れたり、取り扱いに十分ご注意ください。増加の接触は、動物を刺激することができます。 </li><li>無人ホルダーに動物を離れることはありません。 </li></ol></li><li>カフの配置<ol s極秘にするには、=&quot;パディング：5pxの;&quot;&gt; <li>を通じてスレッド尾を&quot;オクルージョンカフは。&quot;力なく、可能な限り尾の付け根に近いとこのカフを置きます。 </li><li>ホルダーの上部背面のノッチの&quot;オクルージョンカフチューブを&quot;確保する。 </li><li> &quot;オクルージョンカフ&quot;から2mm以内に配置、&quot;VPRカフ&quot;を介して尾を通します。 </li><li>ホルダーの上部背面のノッチの&quot;VPRカフチューブを&quot;確保する。 </li><li> CODAのコントローラに袖口を取り付けます。 </li></ol></li><li>動物の温度<ol style="padding:5px;"><li>少なくとも5分は、体温を調節するために動物を許可する。 </li><li>赤外線温度計で頻繁に動物の温度を記録します。動物の温度は32〜35℃にする必要があります</li></ol></li></ol></li></ol></li><li>実験<ol style="padding:5px;"><li>実験を始める<ol style="padding:5px;"><li> &quot;Finish&quot;をクリックし、実験がすぐに開始されます。 </li></ol></li><li>最後の実験<ol style="padding:5px;"><li>動物<ol style="padding:5px;"><li>すぐに袖口とホルダーから動物を取り外します。 </li><li> &quot;シンプルセッションサマリーレポート&quot;が表示された後にケージに動物を返します。 </li></ol></li><li>データ収集<ol style="padding:5px;"><li>受理サイクルが自動的にCODAアプリケーション内のス​​プレッドシート形式で表示される最後のサイクル終了後、&quot;Excelにエクスポート&quot;ボタンをクリックしてください。 </li><li> 、でファイルを配置するフォルダを選択し、ファイルを名前を付けて&quot;保存&quot;をクリックします。 </li><li> Excelアプリケーションを開いて、&quot;開く&quot;をクリックしてください。 </li><li> &quot;保存された。csvファイルを&quot;表示するすべてのファイルタイプを表示する、ファイルをクリックしてください。 </li></ol></li><li>データ処理<ol style="padding:5px;"><li> Excelファイル内のデータを処理するために、いくつかの方法があります。一般的な方法は、平均値と標準偏差を取得することです。 </li><li>標準偏差が30より大きい場合の測定値を削除します。 </li><li>また、拒否されたサイクルが見られるかもしれないと、データベース全体を簡単にExcelにエクスポートすることができます。詳細については、ユーザーズマニュアルを参照してください。 </li></ol></li></ol></li></ol></li></ol>

The authors have nothing to disclose.

measuring blood pressure in mice using volume pressure recording, a tail-cuff method

CODA 8チャンネル高スループット非観血式血圧のシステムは、同時に最大8匹のマウスまたはラットの血圧を測定します。 CODAテールカフシステムは、尾の血液量を決定することにより血圧を測定するためにボリュームプレッシャーレコーディング（VPR）を使用しています。個々のパルス信号を取得することなく、特別に設計された差圧変換器と閉塞尾袖口の測定尾の総血液量。特別な注意は、最も正確な血圧測定値を導出するために、閉塞のカフの長さに与えられる。 VPRは簡単にそのようなC57BL6マウスのような浅黒い肌げっ歯類、上に測定値を取得し、互換性のあるMRIですできます。 CODAのシステムは、6（6）別の血圧パラメータの測定をご提供します。収縮期および拡張期血圧、心拍数、血圧、尾の血流、および尾部の血液量を意味する。測定は、目を覚ましたり、麻酔したマウスまたはラットのいずれかで行うことができます。 CODAのシステムは、コントローラ、ラップトップコンピュータ、ソフトウェア、袖口、動物の所有者、赤外線加温パッド、および赤外線温度計が含まれています。 900グラムと同じ大きさのラットに8グラムのような小さなマウスのための7つの異なるホルダーのサイズがあります。

Watch this Scientific Journal Video about Measuring Blood Pressure in Mice using Volume Pressure Recording, a Tail-cuff Method at JoVE.com

Measuring Blood Pressure in Mice using Volume Pressure Recording, a Tail-cuff Method

CODA 8チャンネル高スループット非観血式血圧のシステムは、同時に最大8匹のマウスまたはラットの血圧を測定します。このテールカフシステムは、尾の血液量を決定することにより血圧を測定するためにボリュームプレッシャーレコーディング（VPR）を使用しています。

ボリューム圧力記録、テールカフ法を用いたマウスにおける測定血圧

The CODA 8-Channel High Throughput Non-Invasive Blood Pressure system measures the blood pressure in up to 8 mice or rats simultaneously. The CODA ...

measuring-blood-pressure-mice-using-volume-pressure-recording-tail

Research

JoVE Journal

Biology

51.3K ビュー.  University of Kentucky. CODA 8チャンネル高スループット非観血式血圧のシステムは、同時に最大8匹のマウスまたはラットの血圧を測定します。このテールカフシステムは、尾の血液量を決定することにより血圧を測定するためにボリュームプレッシャーレコーディング（VPR）を使用しています。

ビデオ: Measuring Blood Pressure in Mice using Volume Pressure Recording, a Tail-cuff Method

Pressure-polishing Pipettes for Improved Patch-clamp Recording

Pressure-polishing is a method for shaping glass pipettes for patch-clamp recording. We first developed this method for fabricating pipettes suitable for recording from small (&lt;3 m) neuronal cell bodies. The basic principal is similar to glass-blowing and combines air pressure and heat to modify the shape of patch pipettes prepared by a conventional micropipette puller. It can be applied to so-called soft (soda lime) and hard (borosilicate) glasses. Generally speaking, pressure polishing can reduce pipette resistance by 25% without decreasing the diameter of the tip opening (Goodman and Lockery, 2000). It can be applied to virtually any type of glass and requires only the addition of a high-pressure valve and fitting to a microforge. This technique is essential for recording from ultrasmall cells (&lt;5 m) and can also improve single-channel recording by minimizing pipette resistance. The blunt shape is also useful for perforated-patch clamp recording since this tip shape results in a larger membrane bleb available for perforation.

This is a guide to modifying the shape of glass micropipettes. Specifically, by using heat and air pressure the taper is widened without increasing the tip opening, leading to lower pipette resistance. This is critical to obtain low noise recordings of small cells but is useful in many applications.

改善されたパッチクランプ記録のための圧力 - 研磨ピペット

Pressure-polishing is a method for shaping glass pipettes for patch-clamp recording.  We first developed this method for fabricating pipettes suitable for ...

生物学

Making Gynogenetic Diploid Zebrafish by Early Pressure

Heterozygosity in diploid eukaryotes often makes genetic studies cumbersome. Methods that produce viable homozygous diploid offspring directly from heterozygous females allow F1 mutagenized females to be screened directly for deleterious mutations in an accelerated forward genetic screen. Streisinger et al.1,2 described methods for making gynogenetic (homozygous) diploid zebrafish by activating zebrafish eggs with ultraviolet light-inactivated sperm and preventing either the second meiotic or the first zygotic cell division using physical treatments (heat or pressure) that deploymerize microtubules. The "early pressure" (EP) method blocks the meiosis II, which occurs shortly after fertilization. The EP method produces a high percentage of viable embryos that can develop to fertile adults of either sex. The method generates embryos that are homozygous at all loci except those that were separated from their centromere by recombination during meiosis I. Homozygous mutations are detected in EP clutches at between 50% for centromeric loci and less than 1% for telomeric loci. This method is reproduced verbatim from the Zebrafish Book3.

This is a method for generating gynogenetic diploid zebrafish embryos (embryos whose only genetic contribution comes from the mother) by blocking the second meiotic division immediately after fertilization with ultraviolet light-inactivated sperm. EP embryos are not fully homozygous due to recombination during the first meiotic division, however they are homozygous at all loci that have not been separated from their centromere by recombination.

初期の圧力による雌性発生二倍体のゼブラフィッシュを作成

Heterozygosity in diploid eukaryotes often makes genetic studies cumbersome. Methods that produce viable homozygous diploid offspring directly from ...

Ambulatory ECG Recording in Mice

Telemetric ECG recording in mice is essential to understanding the mechanisms behind arrhythmias, conduction disorders, and sudden cardiac death. Although the surface ECG is utilized for short-term measurements of waveform intervals, it is not practical for long-term studies of heart rate variability or the capture of rare episodes of arrhythmias. Implantable ECG telemeters offer the advantages of simple surgical implantation, long-term recording of electrograms in ambulatory mice, and scalability with simultaneous recordings of multiple animals. Here, we present a step-by-step guide to the implantation of telemeters for ambulatory ECG recording in mice. Careful adherence to aseptic technique is required for favorable survival results with the possibility of implantation and recording from weeks to months. Thus, implantable ECG telemetry is a valuable tool for detection of critical information on cardiac electrophysiology in ambulatory animal models such as the mouse. 

Telemetric ECG has emerged as an essential tool in evaluating animal models for cardiac arrhythmias and sudden cardiac death. Here, we present a stepwise guide to telemetric ECG recordings for application in long-term ambulatory ECG monitoring in mice.

マウスにおけるホルター心電図の記録

Telemetric ECG recording in mice is essential to understanding the mechanisms behind arrhythmias, conduction disorders, and sudden cardiac death. Although ...

Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice

Endothelial cells provide a useful research model in many areas of vascular biology. Since its first isolation 1, human umbilical vein endothelial cells (HUVECs) have shown to be convenient, easy to obtain and culture, and thus are the most widely studied endothelial cells. However, for research focused on processes like angiogenesis, permeability or many others, microvascular endothelial cells (ECs) are a much more physiologically relevant model to study 2. Furthermore, ECs isolated from knockout mice provide a useful tool for analysis of protein function ex vivo. Several approaches to isolate and culture microvascular ECs of different origin have been reported to date 3-7, but consistent isolation and culture of pure ECs is still a major technical problem in many laboratories. Here, we provide a step-by-step protocol on a reliable and relatively simple method of isolating and culturing mouse lung endothelial cells (MLECs). In this approach, lung tissue obtained from 6- to 8-day old pups is first cut into pieces, digested with collagenase/dispase (C/D) solution and dispersed mechanically into single-cell suspension. MLECS are purified from cell suspension using positive selection with anti-PECAM-1 antibody conjugated to Dynabeads using a Magnetic Particle Concentrator (MPC). Such purified cells are cultured on gelatin-coated tissue culture (TC) dishes until they become confluent. At that point, cells are further purified using Dynabeads coupled to anti-ICAM-2 antibody. MLECs obtained with this protocol exhibit a cobblestone phenotype, as visualized by phase-contrast light microscopy, and their endothelial phenotype has been confirmed using FACS analysis with anti-VE-cadherin 8 and anti-VEGFR2 9 antibodies and immunofluorescent staining of VE-cadherin. In our hands, this two-step isolation procedure consistently and reliably yields a pure population of MLECs, which can be further cultured. This method will enable researchers to take advantage of the growing number of knockout and transgenic mice to directly correlate in vivo studies with results of in vitro experiments performed on isolated MLECs and thus help to reveal molecular mechanisms of vascular phenotypes observed in vivo.

Here, we describe a protocol for isolation and culture of murine pulmonary endothelial cells. This method comprises mechanic and enzymatic lung tissue dissociation as well as a 2-step purification process using anti-PECAM-1 and anti-ICAM-2 antibodies conjugated to magnetic beads, which produces a pure endothelial cell population of mostly microvascular origin.

新生児マウスからの肺血管内皮細胞の単離と培養

Endothelial cells provide a useful research model in many areas of vascular biology. Since its first isolation 1, human umbilical vein endothelial cells ...

Assessing Murine Resistance Artery Function Using Pressure Myography

Pressure myograph systems are exquisitely useful in the functional assessment of small arteries, pressurized to a suitable transmural pressure. The near physiological condition achieved in pressure myography permits in-depth characterization of intrinsic responses to pharmacological and physiological stimuli, which can be extrapolated to the in vivo behavior of the vascular bed. Pressure myograph has several advantages over conventional wire myographs. For example, smaller resistance vessels can be studied at tightly controlled and physiologically relevant intraluminal pressures. Here, we study the ability of 3rd order mesenteric arteries (3-4 mm long), preconstricted with phenylephrine, to vaso-relax in response to acetylcholine. Mesenteric arteries are mounted on two cannulas connected to a pressurized and sealed system that is maintained at constant pressure of 60 mmHg. The lumen and outer diameter of the vessel are continuously recorded using a video camera, allowing real time quantification of the vasoconstriction and vasorelaxation in response to phenylephrine and acetylcholine, respectively.
	To demonstrate the applicability of pressure myography to study the etiology of cardiovascular disease, we assessed endothelium-dependent vascular function in a murine model of systemic hypertension. Mice deficient in the &alpha;1 subunit of soluble guanylate cyclase (sGC&alpha;1-/-) are hypertensive when on a 129S6 (S6) background (sGC&alpha;1-/-S6) but not when on a C57BL/6 (B6) background (sGC&alpha;1-/-B6). Using pressure myography, we demonstrate that sGC&alpha;1-deficiency results in impaired endothelium-dependent vasorelaxation. The vascular dysfunction is more pronounced in sGC&alpha;1-/-S6 than in sGC&alpha;1-/-B6 mice, likely contributing to the higher blood pressure in sGC&alpha;1-/-S6 than in sGC&alpha;1-/-B6 mice. 
	Pressure myography is a relatively simple, but sensitive and mechanistically useful technique that can be used to assess the effect of various stimuli on vascular contraction and relaxation, thereby augmenting our insight into the mechanisms underlying cardiovascular disease.

In pressure myography, an intact small segment of a vessel is mounted onto two small cannulas and pressurized to a suitable luminal pressure. Here, we describe the method to measure vasorelaxation response of the mouse 3rd order mesenteric arteries in c57 and sGC&alpha;1-/- mice using pressure myography.

圧力筋運動記録法を用いたマウス抵抗動脈機能を評価

Pressure myograph systems are  exquisitely useful in the functional assessment of small arteries, pressurized  to a suitable transmural pressure. The  ...

One-channel Cell-attached Patch-clamp Recording

Ion channel proteins are universal devices for fast communication across biological membranes. The temporal signature of the ionic flux they generate depends on properties intrinsic to each channel protein as well as the mechanism by which it is generated and controlled and represents an important area of current research. Information about the operational dynamics of ion channel proteins can be obtained by observing long stretches of current produced by a single molecule. Described here is a protocol for obtaining one-channel cell-attached patch-clamp current recordings for a ligand gated ion channel, the NMDA receptor, expressed heterologously in HEK293 cells or natively in cortical neurons. Also provided are instructions on how to adapt the method to other ion channels of interest by presenting the example of the mechano-sensitive channel PIEZO1. This method can provide data regarding the channel&#8217;s conductance properties and the temporal sequence of open-closed conformations that make up the channel&#8217;s activation mechanism, thus helping to understand their functions in health and disease.

Described here is a procedure for obtaining long stretches of current recording from one ion channel with the cell-attached patch-clamp technique. This method allows for observing, in real time, the pattern of open-close channel conformations that underlie the biological signal. These data inform about channel properties in undisturbed biological membranes.

一チャネルセル付パッチクランプ法

Ion channel proteins are universal devices for fast communication across biological membranes. The temporal signature of the ionic flux they generate ...

Measuring Respiratory Function in Mice Using Unrestrained Whole-body Plethysmography

Respiratory dysfunction is one of the leading causes of morbidity and mortality in the world and the rates of mortality continue to rise. Quantitative assessment of lung function in rodent models is an important tool in the development of future therapies. Commonly used techniques for assessing respiratory function including invasive plethysmography and forced oscillation. While these techniques provide valuable information, data collection can be fraught with artefacts and experimental variability due to the need for anesthesia and/or invasive instrumentation of the animal. In contrast, unrestrained whole-body plethysmography (UWBP) offers a precise, non-invasive, quantitative way by which to analyze respiratory parameters. This technique avoids the use of anesthesia and restraints, which is common to traditional plethysmography techniques. This video will demonstrate the UWBP procedure including the equipment set up, calibration and lung function recording. It will explain how to analyze the collected data, as well as identify experimental outliers and artefacts that results from animal movement. The respiratory parameters obtained using this technique include tidal volume, minute volume, inspiratory duty cycle, inspiratory flow rate and the ratio of inspiration time to expiration time. UWBP does not rely on specialized skills and is inexpensive to perform. A key feature of UWBP, and most appealing to potential users, is the ability to perform repeated measures of lung function on the same animal.

The assessment of respiratory physiology has traditionally relied upon techniques, which require restraint or sedation of the animal. Unrestrained whole-body plethysmography, however, provides precise, non-invasive, quantitative analysis of respiratory physiology in animal models. In addition, the technique allows repeated respiratory assessment of mice allowing for longitudinal studies.

拘束されていない全身プレチスモグラフィを使用したマウスにおける呼吸機能の測定

Respiratory dysfunction is one of the leading causes of morbidity and mortality in the world and the rates of mortality continue to rise. Quantitative ...

A Method for Screening and Validation of Resistant Mutations Against Kinase Inhibitors

The discovery of BCR/ABL as a driver oncogene in chronic myeloid leukemia (CML) resulted in the development of Imatinib, which, in fact, demonstrated the potential of targeting the kinase in cancers by effectively treating the CML patients. This observation revolutionized drug development to target the oncogenic kinases implicated in various other malignancies, such as, EGFR, B-RAF, KIT and PDGFRs. However, one major drawback of anti-kinase therapies is the emergence of drug resistance mutations rendering the target to have reduced or lost affinity for the drug. Understanding the mechanisms employed by resistant variants not only helps in developing the next generation inhibitors but also gives impetus to clinical management using personalized medicine. We reported a retroviral vector based screening strategy to identify the spectrum of resistance conferring mutations in BCR/ABL, which has helped in developing the next generation BCR/ABL inhibitors. Using Ruxolitinib and JAK2 as a drug target pair, here we describe in vitro screening methods that utilizes the mouse BAF3 cells expressing the random mutation library of JAK2 kinase.

Emergence of genetic resistance against kinase inhibitor therapy poses significant challenge for effective cancer therapy. Identification and characterization of resistant mutations against a newly developed drug helps in better clinical management and next generation drug design. Here, we describe our protocol for in vitro screening and validation of resistant mutations.

キナーゼ阻害剤に対して耐性変異のスクリーニングおよび検証のための方法

The discovery of BCR/ABL as a driver oncogene in chronic myeloid leukemia (CML) resulted in the development of Imatinib, which, in fact, demonstrated the ...

Cardiac Pressure-Volume Loop Analysis Using Conductance Catheters in Mice

Cardiac pressure-volume loop analysis is the &#8220;gold-standard&#8221; in the assessment of load-dependent and load-independent measures of ventricular systolic and diastolic function. Measures of ventricular contractility and compliance are obtained through examination of cardiac response to changes in afterload and preload. These techniques were originally developed nearly three decades ago to measure cardiac function in large mammals and humans. The application of these analyses to small mammals, such as mice, has been accomplished through the optimization of microsurgical techniques and creation of conductance catheters. Conductance catheters allow for estimation of the blood pool by exploiting the relationship between electrical conductance and volume. When properly performed, these techniques allow for testing of cardiac function in genetic mutant mouse models or in drug treatment studies. The accuracy and precision of these studies are dependent on careful attention to the calibration of instruments, systematic conduct of hemodynamic measurements and data analyses. We will review the methods of conducting pressure-volume loop experiments using a conductance catheter in mice.

Cardiac pressure-volume loop analysis is the most comprehensive way to measure cardiac function in the intact heart. We describe a technique to perform and analyze cardiac pressure volume loops, using conductance catheters.

マウスにおけるコンダクタンスカテーテルを用いて心臓圧力 - 体積ループ解析

Cardiac pressure-volume loop analysis is the &#8220;gold-standard&#8221; in the assessment of load-dependent and load-independent measures of ventricular ...

Measuring Pressure Volume Loops in the Mouse

Understanding the causes and progression of heart disease presents a significant challenge to the biomedical community. The genetic flexibility of the mouse provides great potential to explore cardiac function at the molecular level. The mouse&#39;s small size does present some challenges in regards to performing detailed cardiac phenotyping. Miniaturization and other advancements in technology have made many methods of cardiac assessment possible in the mouse. Of these, the simultaneous collection of pressure and volume data provides a detailed picture of cardiac function that is not available through any other modality. Here a detailed procedure for the collection of pressure-volume loop data is described. Included is a discussion of the principles underlying the measurements and the potential sources of error. Anesthetic management and surgical approaches are discussed in great detail as they are both critical to obtaining high quality hemodynamic measurements. The principles of hemodynamic protocol development and relevant aspects of data analysis are also addressed.

This manuscript describes a detailed protocol for the collection of pressure-volume data from the mouse.

マウスで圧力ボリュームループを測定します

Understanding the causes and progression of heart disease presents a significant challenge to the biomedical community. The genetic flexibility of the ...