<a href="https://www.jove.com/video/50255">肉のスライスせん断力を分析するための角度調整可能なカッティングボックスをご紹介します</a> <a href="/author/Tom_Whitesell" rel="author">トム·ホワイトセル</a> 1、 <a href="/author/Carmen_Avil%E9s" rel="author">カルメンアビレス</a> 2、 <a href="/author/Jennifer+L._Aalhus" rel="author">ジェニファーL. Aalhus</a> 1、 <a href="/author/Chris+R._Calkins" rel="author">クリスR.カルキン</a> 3、 <a href="/author/Ivy+L._Larsen" rel="author">アイビーL.ラーセン</a> 1、 <a href="/author/Manuel_Ju%E1rez" rel="author">マヌエル·フアレス</a> 1 スライス剪断力は、牛肉のテクスチャ解析のための参照方法である。角度調整可能な切削ボックスを使用して、研究目的のためにその精度を高めることができる。 最長筋内の別の場所からの結果は、ワーナー·Bratzlerせん断力法と高い相関を示してと異なる筋肉の可能性が高い適応性。 <a href="https://www.jove.com/video/50331">人間の皮膚生検における侵害受容性表皮内神経線維の三次元イメージング</a> <a href="/author/Jacqueline_Dauch" rel="author">ジャクリーンDauch</a> 1、 <a href="/author/Chelsea_Lindblad" rel="author">チェルシーリンドブラッド</a> 1、 <a href="/author/John+M._Hayes" rel="author">ジョンM.ヘイズ</a> 1、 <a href="/author/Stephen+I._Lentz" rel="author">スティーブンI.レンツ</a> 2、 <a href="/author/Hsinlin+T._Cheng" rel="author">Hsinlin T.チェン</a> 1 痛みを伴う神経障害（PN）における侵害受容性表皮内神経線維（IENFs）の変化を調べるために、我々が直接侵害受容IENFsで観察された三次元形態学的変化を調べることができるプロトコルを開発した。 IENFsの三次元解析は、形態学的なCHを評価する可能性を秘めている<html> PNでIENFのアンジェ。 <a href="https://www.jove.com/video/50451">携帯電話上の広視野蛍光顕微鏡と蛍光イメージングフローサイトメトリー</a> <a href="/author/Hongying_Zhu" rel="author">朱Hongying</a> 1、 <a href="/author/Aydogan_Ozcan" rel="author">Aydogan Ozcan氏</a> 1,2,3 私たちは、コンパクトでコスト効率の良い光流体添付ファイルを使用して携帯電話上の蛍光顕微鏡とイメージングフローサイトメトリーツールの統合に関する我々の最近の結果を確認します。これらの携帯電話ベースのマイクロ分析装置は、次のようなリソースの限られた設定で、例えば、水サンプルのハイスループットスクリーニングのためだけでなく、様々な細胞カウントタスクを実行するように、フローサイトメトリー解析に役に立つかもしれません。 <a href="https://www.jove.com/video/3194">人間の毛包から神経堤幹細胞の単離と培養</a> feng_Yang &quot;REL =&quot;作成者&quot;&gt; Ruifengヤン、 <a href="/author/Xiaowei_Xu" rel="author">小威徐</a> この記事では、人間の毛包から神経堤幹細胞の単離および培養のための堅牢なプロトコルを提示します。

利害の衝突は宣言されていない。

april 2013: this month in jove

ここでは可視化実験（Joveの）のジャーナルの2013年4月号に出てくるのかを見ていきましょう。 それがために汚染の問題や潜在的に危険な結果の研究室では食べ物を持っていることは通常許されないのです。しかし、今月、Joveのは、ラボで料理ステーキを伴うビデオの記事を提供しています。 <a href="http://www.jove.com/video/50255">ホワイトセルらは</a>牛肉産業における重要な関心事である調理牛肉の優しさを測定するための手法を提案する。ステーキ繊維の角度はせん断力に影響を与える可能性があるため、著者らは、スライスが筋繊維に平行であるので、それらは牛肉をカットすることができる可変角度切断·ボックスを使用する。これは、ステーキをカットするのに必要な最大の力を正確に測定することができます。 別の記事では、我々は牛肉からこのようなフェイスリフトの手続きから残されたものとして、人間の皮膚サンプル、に移動します。毛包コンタ幹細胞は、多くの異なるタイプのため、 <a href="http://www.jove.com/video/3194">Yangと徐</a>皮膚サンプルから毛包を抽出し、蛍光活性化細胞選別（FACS）を用いて神経堤幹細胞（NCSCs）を単離する方法を示す。著者は、その後、潜在的再生医療で使用することができますどのように文化にNCSCs、実証する。 Joveの臨床およびトランスレーショナル医学では、人間の皮膚サンプルを含む別の記事を持っている。によって記述<a href="http://www.jove.com/video/50331">Dauch ら</a>は、この手順では、痛みを伴う神経障害における表皮内神経線維を研究するため、人間の皮膚生検の免疫組織化学を使用しています。三次元イメージングは​​神経障害に関連付けられている形態学的変化（例えば軸索の腫脹など）の可視化を可能にします。これは、医師が正確に皮膚パンチ生検からの小さな繊維神経障害を診断することができます。 最後に、バイオセクションの、我々はすでにJoveの三論文を発表しているUCLA​​のOzcan氏のグループから別の小説顕微鏡技術を持っている。 2012年8月からの最も最近の記事では、マイクロ流体チップ上の画像の白い血液細胞（参照にレンズフリーシステムの利用を実証し<a href="http://www.jove.com/video/4161/lensfree-on-chip-tomographic-microscopy-employing-multi-angle">Isikman ら、2012年</a> ）。グループの第四のJoveの記事では、 <a href="http://www.jove.com/video/50451">朱とOzcan氏は、</a>任意の携帯電話のカメラに光学流体広視野蛍光顕微鏡を添付する方法を示します。液体サンプルは、微小流体チャネルを介して配信され、携帯電話のカメラは、チャネルを流れる細胞のビデオを記録するために使用することができる。この手順では、ポイント·オブ·ケア診断またはフィールドのために非常に有用である可能性がある、蛍光標識された細胞は、小型でコスト効果の高い携帯電話のカメラで撮像され、特徴付けすることができる。 あなただけのHをしました4月のためのJoveののハイライトのいくつかの広告プレビュー。可視化実験のジャーナル：Joveの中でフルレングスの記事を参照するにはウェブサイトに加え、より多くのを、ご覧ください。

Watch this Scientific Journal Video about April 2013: This Month in JoVE at JoVE.com

April 2013: This Month in JoVE

ここでは可視化実験（Joveの）のジャーナルの2013年4月号からのいくつかのハイライトです。

2013年4月：Joveの中で今月

Here's a look at what's coming up in the April 2013 issue of Journal of Visualized Experiments (JoVE).
It's usually not permissible to have food in ...

Without Section

7.7K ビュー.  Massachusetts Eye and Ear. ここでは可視化実験（Joveの）のジャーナルの2013年4月号からのいくつかのハイライトです。

ビデオ: April 2013: This Month in JoVE

August 2012: This Month in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens free lens-free "on-chip" microscopy platform, which they have pioneered.
Back in 2009, the Ozcan lab published their first article, which demonstrated the use of a lens-free imaging platform that could image cells within whole blood. This imaging technique involves placing a sample directly on an optoelectric sensor array, like the CCD or CMOS chips found in the standard camcorder. Then, LED illumination from above the sample casts shadows on the chip, which are recorded as digital holograms that can be subjected to image processing. Not only does this lensless imaging system allow for an extremely large field of view (FOV of 0.6-8 cm2), the lack of bulky optical components makes it excellent for microfluidic applications. The Ozcan group demonstrates this in their follow-up 2011 JoVE article, in which fluorescently labeled white blood cells are imaged within a microfluidic chip.
This August in JoVE, the Ozcan group demonstrates further advancements to their lens-free microscopy platform by applying it to tomographic imaging of the nematode, C. elegans. By changing the angle of illumination, using a motorized rotation stage, the Ozcan group shows how multiple holographic slices may be generated for a sample-which, following processing, can be combined into tomograms. The Ozcan group confirms that their 3D reconstruction of the worm is successful by citing that the pharyngeal tube, a 5-micron-thick structure, is only visible in a single slice and in a 3D reconstructed cross section.
The compact and inexpensive nature of the lens-free imaging systems - designed and assembled by the Ozcan group - make them well suited for point-of-care diagnostic applications, or even cell phone based microscopy that can be performed in the field.
At the Ernest Gallo Clinic and Research Center at University of California, San Francisco JoVE learns how to visualize morphological changes in single cells using three-dimensional fluorescence microscopy and computer analysis. In this procedure, cells are given agonist, antagonist plus agonist, or no treatment, and prepared for immunofluorescence microscopy. Three-dimensional images are acquired with the help of a two-photon laser system, and the fluorescence data are processed on the computer using Imaris and Matlab.
To demonstrate the power of this technique, our authors quantify single-cell phenotypic changes in transfected HEK293 cells. Three-dimensional morphometric analysis shows dramatic changes in cells treated with agonist. These changes are not always apparent when using conventional 2D analysis. This technique allows morphological changes in single cells to be quantified and characterized in response to pharmacologic stimuli, offering a powerful new assessment tool to the arsenal of drug discovery research.
This month, in JoVE Applied Physics, we travel to South Carolina to visit the School of Materials Science and Engineering at Clemson University. Here, researchers show us how to construct lithium batteries for laboratory research.
The basic components of a lithium coin cell are the working electrode, the lithium electrolyte, and a lithium foil counter electrode. The different components of the coin cell are carefully assembled and sealed with a crimping machine. The finished coin cell can then be characterized in a battery tester. Using their homemade coin cells, our researchers can experiment with using different materials to make the cathodes of lithium coin cells.
This month in JoVE Immunology and Infection, we head to Basel, Switzerland to witness two techniques for diagnosing helminth infections. We also see how investigators perform the shuttle run test in children, in order to assess the effects of these infections on physical fitness.
Helminths are parasitic worms that can live inside humans and animals. Many species infect the intestinal tract, and may be transmitted through soil. Our authors demonstrate the Kato-Katz technique, which is used for diagnosing several common soil-transmitted helminths. They also demonstrate the Baermann technique, which uses light to stimulate the migration of certain types of phototactic helminths out of sample preparations. The various types of helminth species can subsequently be determined based on morphology.
Our authors then demonstrate how to set up and perform a 20-meter shuttle run test for children, and to measure the impact of helminth infections on physical fitness. This method can help determine the true burden of soil-transmitted helminth infections on overall health.
This brief summary highlights but a few notable video-articles that will be released in August. We'll also feature articles that demonstrate how to express genes in the midbrain of the chick embryo, study neutrophil transmigration in parallel flow chambers lined with endothelial cells, and precisely measure proteolytic degradation of the extracellular matrix.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

2012年8月：Joveのでは、今月

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and ...

Research

JoVE Journal

生物学

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

2012：In Reviewに年

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...