<a href="https://www.jove.com/video/50255">L&#39;introduction d&#39;une boîte de coupe réglable Angle pour l&#39;analyse Slice Force de cisaillement dans la viande</a> <a href="/author/Tom_Whitesell" rel="author">Tom Whitesell</a> 1, <a href="/author/Carmen_Avil%E9s" rel="author">Carmen Avilés</a> 2, <a href="/author/Jennifer+L._Aalhus" rel="author">Jennifer L. Aalhus</a> 1, <a href="/author/Chris+R._Calkins" rel="author">Chris R. Calkins</a> 3, <a href="/author/Ivy+L._Larsen" rel="author">Ivy L. Larsen</a> 1, <a href="/author/Manuel_Ju%E1rez" rel="author">Manuel Juárez</a> 1 force de cisaillement Slice est une méthode de référence pour l&#39;analyse de la texture de la viande bovine. En utilisant une boîte de coupe réglable angle pourrait augmenter sa précision à des fins de recherche. Les résultats de différents endroits dans le muscle long dorsal montrent une forte corrélation avec la méthode de la force de cisaillement Warner-Bratzleret une grande adaptabilité potentiel de différents muscles. <a href="https://www.jove.com/video/50331">L&#39;imagerie tridimensionnelle de nociceptifs fibres nerveuses intra-épidermiques dans des biopsies de peau humaine</a> <a href="/author/Jacqueline_Dauch" rel="author">Jacqueline Dauch</a> 1, <a href="/author/Chelsea_Lindblad" rel="author">Chelsea Lindblad</a> 1, <a href="/author/John+M._Hayes" rel="author">John M. Hayes</a> 1, <a href="/author/Stephen+I._Lentz" rel="author">Stephen I. Lentz</a> 2, <a href="/author/Hsinlin+T._Cheng" rel="author">Hsinlin T. Cheng</a> 1 Afin d&#39;étudier les changements de fibres nerveuses nociceptives (intraépidermiques IENFs) dans les neuropathies douloureuses (PN), nous avons développé des protocoles qui pourraient examiner directement les modifications morphologiques tridimensionnels observés dans IENFs nociceptifs. Analyse tridimensionnelle de IENFs a le potentiel d&#39;évaluer le ch morphologique<html> Anges de IENF en PN. <a href="https://www.jove.com/video/50451">Microscopie à fluorescence à champ large et Fluorescent cytométrie de flux d&#39;imagerie sur une cellule-téléphone</a> <a href="/author/Hongying_Zhu" rel="author">Hongying Zhu</a> 1, <a href="/author/Aydogan_Ozcan" rel="author">Aydogan Ozcan</a> 1,2,3 Nous passons en revue nos résultats récents sur l&#39;intégration de la microscopie à fluorescence et des outils de cytométrie de flux d&#39;imagerie sur un téléphone portable à l&#39;aide des pièces jointes opto-fluidiques compacts et rentables. Dispositifs micro-analyses fondées Ce téléphone portable pourraient être utiles pour l&#39;analyse cytométrique, comme la réalisation de diverses tâches de comptage de cellules ainsi que pour le criblage à haut débit de, par exemple, des échantillons d&#39;eau dans les pays à ressources limitées. <a href="https://www.jove.com/video/3194">Isolement et culture des cellules souches neurales de crête à partir des follicules de cheveux humains</a> feng_Yang &quot;rel =&quot; author &quot;&gt; Ruifeng Yang, <a href="/author/Xiaowei_Xu" rel="author">Xu Xiaowei</a> Cet article présente un protocole robuste pour l&#39;isolement et la culture des cellules souches de la crête neurale des follicules pileux humains.

Aucun conflit d&#39;intérêt déclaré.

april 2013: this month in jove

Voici un aperçu de ce qui s&#39;en vient dans le numéro d&#39;Avril 2013 du Journal of Experiments Visualized (JoVE). Il n&#39;est généralement pas permis d&#39;avoir de la nourriture dans les laboratoires de recherche en raison de problèmes de contamination et les conséquences potentiellement dangereuses. Cependant, ce mois-ci, JoVE dispose d&#39;un vidéo-article qui implique faisant cuire le bifteck dans le laboratoire. <a href="http://www.jove.com/video/50255">Whitesell et al.</a> présenter une méthode pour mesurer la tendresse de boeuf cuit, qui est une préoccupation importante dans l&#39;industrie du bœuf. Parce que l&#39;angle des fibres de steak peut affecter la force de cisaillement, les auteurs utilisent une boîte de coupe à angle variable, ce qui leur permet de réduire le boeuf si la tranche est parallèle aux fibres musculaires. Cela permet des mesures précises de la force maximale nécessaire pour couper un steak. Dans un autre article, nous passons de la viande à des échantillons de peau humaine, tels que ceux laissés par les procédures de lifting. Les follicules pileux contadans de nombreux types de cellules souches, de sorte <a href="http://www.jove.com/video/3194">Yang Xu et</a> montrent comment extraire les follicules pileux à partir d&#39;échantillons de peau et d&#39;isoler les cellules souches de la crête neurale (SNCLC) par tri cellulaire activé par fluorescence (FACS). Les auteurs montrent ensuite comment la culture des SNCLC, qui peuvent potentiellement être utilisés dans la médecine régénérative. En médecine clinique et translationnelle JoVE, nous avons un autre article impliquant des échantillons de peau humaine. Décrit par <a href="http://www.jove.com/video/50331">Dauch et al.</a> , cette procédure utilise immunohistochimie sur des biopsies de peau humaine pour étudier les fibres nerveuses intraépidermiques dans neuropathie douloureuse. L&#39;imagerie tridimensionnelle permet la visualisation des changements morphologiques (par exemple, des gonflements axonaux) associés aux neuropathies. Cela permet aux médecins de diagnostiquer avec précision les petits neuropathies de fibres provenant de biopsies de perforation de la peau. Enfin, dans la section bio-ingénierie, Nous avons une autre technique de microscopie roman du groupe Ozcan à UCLA, qui ont déjà publié trois articles dans Jupiter. Leur article le plus récent, à partir de Août 2012 a démontré l&#39;utilisation d&#39;un système de lentilles de droits à l&#39;image des globules blancs sur une puce microfluidique (voir <a href="http://www.jove.com/video/4161/lensfree-on-chip-tomographic-microscopy-employing-multi-angle">Isikman et al. 2012</a> ). En quatrième article de JoVE du groupe, <a href="http://www.jove.com/video/50451">Zhu et Ozcan</a> montrent comment attacher un microscope à fluorescence à champ large opto-fluidique à une caméra de téléphone cellulaire. Les échantillons liquides sont livrées via un canal microfluidique, et la caméra d&#39;un téléphone cellulaire peuvent être utilisés pour enregistrer une vidéo des cellules circulant dans le canal. Cette procédure permet aux cellules marquées par fluorescence à imager et caractérisés par une caméra de téléphone cellulaire compact et rentable, qui pourrait être très utile pour le point-of-care ou le champ des diagnostics. Vous venez de hannonce un aperçu de quelques-uns des faits saillants de Jupiter pour le mois d&#39;Avril. Visitez le site pour voir les articles complets, ainsi que plusieurs autres, dans JoVE: The Journal of Experiments visualisé.

Watch this Scientific Journal Video about April 2013: This Month in JoVE at JoVE.com

April 2013: This Month in JoVE

Voici quelques faits saillants du Avril 2013 Autorisation d&#39;émettre des Journal of Experiments Visualized (JoVE).

Avril 2013: Ce mois-ci dans JoVE

Here's a look at what's coming up in the April 2013 issue of Journal of Visualized Experiments (JoVE).
It's usually not permissible to have food in ...

Without Section

7.7K vues.  Massachusetts Eye and Ear. Voici quelques faits saillants du Avril 2013 Autorisation d'émettre des Journal of Experiments Visualized (JoVE).

Vidéo: April 2013: This Month in JoVE

August 2012: This Month in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens free lens-free "on-chip" microscopy platform, which they have pioneered.
Back in 2009, the Ozcan lab published their first article, which demonstrated the use of a lens-free imaging platform that could image cells within whole blood. This imaging technique involves placing a sample directly on an optoelectric sensor array, like the CCD or CMOS chips found in the standard camcorder. Then, LED illumination from above the sample casts shadows on the chip, which are recorded as digital holograms that can be subjected to image processing. Not only does this lensless imaging system allow for an extremely large field of view (FOV of 0.6-8 cm2), the lack of bulky optical components makes it excellent for microfluidic applications. The Ozcan group demonstrates this in their follow-up 2011 JoVE article, in which fluorescently labeled white blood cells are imaged within a microfluidic chip.
This August in JoVE, the Ozcan group demonstrates further advancements to their lens-free microscopy platform by applying it to tomographic imaging of the nematode, C. elegans. By changing the angle of illumination, using a motorized rotation stage, the Ozcan group shows how multiple holographic slices may be generated for a sample-which, following processing, can be combined into tomograms. The Ozcan group confirms that their 3D reconstruction of the worm is successful by citing that the pharyngeal tube, a 5-micron-thick structure, is only visible in a single slice and in a 3D reconstructed cross section.
The compact and inexpensive nature of the lens-free imaging systems - designed and assembled by the Ozcan group - make them well suited for point-of-care diagnostic applications, or even cell phone based microscopy that can be performed in the field.
At the Ernest Gallo Clinic and Research Center at University of California, San Francisco JoVE learns how to visualize morphological changes in single cells using three-dimensional fluorescence microscopy and computer analysis. In this procedure, cells are given agonist, antagonist plus agonist, or no treatment, and prepared for immunofluorescence microscopy. Three-dimensional images are acquired with the help of a two-photon laser system, and the fluorescence data are processed on the computer using Imaris and Matlab.
To demonstrate the power of this technique, our authors quantify single-cell phenotypic changes in transfected HEK293 cells. Three-dimensional morphometric analysis shows dramatic changes in cells treated with agonist. These changes are not always apparent when using conventional 2D analysis. This technique allows morphological changes in single cells to be quantified and characterized in response to pharmacologic stimuli, offering a powerful new assessment tool to the arsenal of drug discovery research.
This month, in JoVE Applied Physics, we travel to South Carolina to visit the School of Materials Science and Engineering at Clemson University. Here, researchers show us how to construct lithium batteries for laboratory research.
The basic components of a lithium coin cell are the working electrode, the lithium electrolyte, and a lithium foil counter electrode. The different components of the coin cell are carefully assembled and sealed with a crimping machine. The finished coin cell can then be characterized in a battery tester. Using their homemade coin cells, our researchers can experiment with using different materials to make the cathodes of lithium coin cells.
This month in JoVE Immunology and Infection, we head to Basel, Switzerland to witness two techniques for diagnosing helminth infections. We also see how investigators perform the shuttle run test in children, in order to assess the effects of these infections on physical fitness.
Helminths are parasitic worms that can live inside humans and animals. Many species infect the intestinal tract, and may be transmitted through soil. Our authors demonstrate the Kato-Katz technique, which is used for diagnosing several common soil-transmitted helminths. They also demonstrate the Baermann technique, which uses light to stimulate the migration of certain types of phototactic helminths out of sample preparations. The various types of helminth species can subsequently be determined based on morphology.
Our authors then demonstrate how to set up and perform a 20-meter shuttle run test for children, and to measure the impact of helminth infections on physical fitness. This method can help determine the true burden of soil-transmitted helminth infections on overall health.
This brief summary highlights but a few notable video-articles that will be released in August. We'll also feature articles that demonstrate how to express genes in the midbrain of the chick embryo, study neutrophil transmigration in parallel flow chambers lined with endothelial cells, and precisely measure proteolytic degradation of the extracellular matrix.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

Août 2012: Ce mois-ci dans JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and ...

Recherche

JoVE Journal

Biologie

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

2012: Une année en revue

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...