質量分析法による総合的なO-結合およびスルホグライコミクスのための改良されたゲル内還元β脱離

1:16

Gel Excision and Removal of Gel-derived Contaminants

3:14

In-gel O-glycan Release by Reductive β-Elimination and Desalting on Cation-exchange Chromatography

6:02

Borate Removal, C18 Clean-up and Base Preparation for Permethylation

8:03

Permethylation of Released Glycans C18 Clean-up and Mass Spectrometry Analysis

10:33

Results: Recovery and Mass Spectral Analysis of Permethylated O-linked Glycans

12:30

Conclusion