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JoVE Journal

Immunology and Infection

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A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue

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09:42 min

June 7th, 2017

June 7th, 2017

11,236 Views

0:05

Title

1:18

Preparation of Lacrimal Gland Extract

2:32

Preparation of Emulsion

3:30

Immunization of Lewis Rats and Injection of Antigen Mixture into the Forniceal Subconjunctiva and Lacrimal Gland

4:53

Assessment of Dry Eye Features

7:19

Results

8:57

Conclusion

文字起こし

This report describes a method to induce chronic experimental autoimmune dry eye in Lewis rats through immunization with an emulsion of rat lacrimal gland extract, ovalbumin, and complete Freund's adjuvant, followed by the injection of lacrimal gland extract and ovalbumin into the forniceal subconjunctiva and lacrimal glands six weeks later.

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