The non-salary component of the work was funded by the Commonwealth of Australia. The author from the University of Delaware gratefully acknowledges the support of the US National Science Foundation (Grant #1254540, Dr. Mary Toney, Program Director). The authors thank Mr. Mark Fitzgerald for his assistance with the electrophoretic deposition measurements.

1. Functionalization of CNTs and GNPs by Ultrasonic Ozonolysis
<ol>
	<li>Weigh the nanomaterials in a glove box inside a HEPA filter-equipped fume cupboard. Weigh the desired quantity of nanomaterials into a beaker. Transfer to a bottle and add ultrapure water to make a concentration of 1 g/L.</li>
	<li>Seal the bottle with a lid. Ultrasonicate in a standard ultrasonic bath (see Materials List; frequency: ~43 &#177;2 kHz; power: 60 W) to disperse the CNTs or GNPs. 
	NOTE: CAUTION. See the Work Health and Safety No...

<ol>
	<li>Pandey, G., Thostenson, E. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Carbon+Nanotube-Based+Multifunctional+Polymer+Nanocomposites.">Carbon Nanotube-Based Multifunctional Polymer Nanocomposites.</a> Polym. Rev. 52 (3), 355-416 (2012).</li><li>Das, T. K., Prusty, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Graphene-Based+Polymer+Composites+and+Their+Applications.">Graphene-Based Polymer Composites and Their Applications.</a> Polym.-Plast. Technol. 52 (4), 319-331 (2013).</li><li>Karger-Kocsis, J., Mahmood, H., Pegoretti, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recent+advances+in+fiber/matrix+interphase+engineering+for+polymer+composites.">Recent advances in fiber/matrix interphase engineering for polymer composites.</a> Prog. Mater. Sci. 73, 1-43 (2015).</li><li>Qian, H., Greenhalgh, E. S., Shaffer, M. S. P., Bismarck, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Carbon+nanotube-based+hierarchical+composites:+a+review.">Carbon nanotube-based hierarchical composites: a review.</a> J. Mater. Chem. 20 (23), 4751-4762 (2010).</li><li>Hummers, W. S., Offeman, R. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preparation+of+graphitic+oxide.">Preparation of graphitic oxide.</a> J. Am. Chem. Soc. 80, 1339-1339 (1958).</li><li>Shaffer, M. S. P., Fan, X., Windle, A. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dispersion+and+Packing+of+Carbon+Nanotubes.">Dispersion and Packing of Carbon Nanotubes.</a> Carbon. 36 (11), 1603-1612 (1998).</li><li>Hamon, M. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dissolution+of+Single-Walled+Carbon+Nanotubes.">Dissolution of Single-Walled Carbon Nanotubes.</a> Adv. Mater. 11, 834-840 (1999).</li><li>Low, C. T. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrochemical+approaches+to+the+production+of+graphene+flakes+and+their+potential+applications.">Electrochemical approaches to the production of graphene flakes and their potential applications.</a> Carbon. 54, 1-21 (2013).</li><li>Rider, A. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hierarchical+composites+with+high-volume+fractions+of+carbon+nanotubes:+Influence+of+plasma+surface+treatment+and+thermoplastic+nanophase-modified+epoxy.">Hierarchical composites with high-volume fractions of carbon nanotubes: Influence of plasma surface treatment and thermoplastic nanophase-modified epoxy.</a> Carbon. 94, 971-981 (2015).</li><li>Tchoul, M. N., Ford, W. T., Lolli, G., Resasco, D. E., Arepalli, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+Mild+Nitric+Acid+Oxidation+on+Dispersability,+Size,+and+Structure+of+Single-Walled+Carbon+Nanotubes.">Effect of Mild Nitric Acid Oxidation on Dispersability, Size, and Structure of Single-Walled Carbon Nanotubes.</a> Chem. Mater. 19, 5765-5772 (2007).</li><li>Gong, X., Liu, J., Baskaran, S., Voise, R. D., Young, J. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surfactant-Assisted+Processing+of+Carbon+Nanotube/Polymer+Composites.">Surfactant-Assisted Processing of Carbon Nanotube/Polymer Composites.</a> Chem. Mater. 12, 1049-1052 (2000).</li><li>An, Q., Rider, A. N., Thostenson, E. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrophoretic+deposition+of+carbon+nanotubes+onto+carbon-fiber+fabric+for+production+of+carbon/epoxy+composites+with+improved+mechanical+properties.">Electrophoretic deposition of carbon nanotubes onto carbon-fiber fabric for production of carbon/epoxy composites with improved mechanical properties.</a> Carbon. 50 (11), 4130-4143 (2012).</li><li>An, Q., Rider, A. N., Thostenson, E. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heirarchical+composite+structures+prepared+by+electrophoretic+deposition+of+carbon+nanotubes+onto+glass+fibers.">Heirarchical composite structures prepared by electrophoretic deposition of carbon nanotubes onto glass fibers.</a> ACS Appl. Mater. Interfac. 5 (6), 2022-2032 (2013).</li><li>Rider, A. N., An, Q., Thostenson, E. T., Brack, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasonicated-ozone+modification+of+exfoliated+graphite+for+stable+aqueous+graphitic+nanoplatelet+dispersions.">Ultrasonicated-ozone modification of exfoliated graphite for stable aqueous graphitic nanoplatelet dispersions.</a> Nanotechnology. 25 (49), 495607 (2014).</li><li>Fairley, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> CasaXPS Manual 2.3.15 Introduction to XPS and AES, Rev. 1.2. , (2009).</li><li>Leiro, J., Heinonen, M., Laiho, T., Batirev, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Core-level+XPS+spectra+of+fullerene,+highly+oriented+pyrolitic+graphite,+and+glassy+carbon.">Core-level XPS spectra of fullerene, highly oriented pyrolitic graphite, and glassy carbon.</a> J. Electron Spectrosc. 128, 205-213 (2003).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> DXR Raman Instruments: Getting Started. , (2008).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Cressington 208HR High Resolution Sputter Coater for FE-SEM: Operating Manual. , (2003).</li><li>Krishnamoorthy, K., Veerapandian, M., Yun, K., Kim, S. -. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+chemical+and+structural+analysis+of+graphene+oxide+with+different+degrees+of+oxidation.">The chemical and structural analysis of graphene oxide with different degrees of oxidation.</a> Carbon. 53, 38-49 (2013).</li><li>Hamaker, H. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Formation+of+a+Deposit+by+Electrophoresis.">Formation of a Deposit by Electrophoresis.</a> T. Faraday Soc. 35, 279-287 (1940).</li><li>Rider, A. N., An, Q., Brack, N., Thostenson, E. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Polymer+nanocomposite+-+fiber+model+interphases:+Influence+of+processing+and+interface+chemistry+on+mechanical+performance.">Polymer nanocomposite - fiber model interphases: Influence of processing and interface chemistry on mechanical performance.</a> Chem. Eng. J. 269, 121-134 (2015).</li></ol>

The authors have nothing to disclose.

When working with nanoparticles of high hardness, such as CNTs, the potential erosion effect on containers and tubing should not be overlooked. Step 1.14 in the protocol was inserted after the tubing became worn at a bend due to CNTs impinging on the tube side wall, causing a system leak.
Also, note that the CNTs are in suspension, not solution, and that they must be stirred before each use if a homogeneous suspension is desired. For example, this would be necessary to maintain the desired con...

The use of carbon nanomaterials to modify polymeric and composite systems has seen intensive research interest over the past 20 years. Recent reviews on both the use of carbon nanotubes1 (CNTs) and graphene nanoplatelets2 (GNPs) provide an indication of the breadth of research. The high specific stiffness and strength of CNTs and GNPs, as well as their high intrinsic electrical conductivity, make the materials ideally suited for incorporation into polymeric systems to enhance both the mechanical and electrical performance of the nanocomposite materials. CNTs and GNPs have also been used for the development of hierarchical composite structures by using the carbon nanomaterials to modify both fiber interfacial adhesion and matrix stiffness3,4.
The homogeneous dispersion of carbon nanomaterials into polymeric systems often requires processing steps, which chemically alter the nanomaterials to improve the chemical compatibility with the polymer matrix, remove impurities, and reduce or remove agglomerates from the as-received materials. A variety of methods to chemically modify carbon nanomaterials are available and may include wet chemical oxidation using strong acids5,6, modification with surfactants7, electrochemical intercalation and exfoliation8, or dry chemical processing using plasma-based processes9.
The use of strong acids in the oxidation step of CNTs introduces oxygen functional groups and removes impurities. However, it has the disadvantage of significantly reducing the CNT length, introducing damage to the CNT outer walls and using dangerous chemicals, which need to be isolated from the treated material for further processing10. The use of surfactants combined with ultrasonication offers a less aggressive method to prepare stable dispersions, but the surfactant is often difficult to remove from the treated material and may not be compatible with the polymer being used to prepare the nanocomposite materials1,11. The strength of the chemical interaction between the surfactant molecule and CNT or GNP may also be insufficient for mechanical applications. Dry plasma treatment processes conducted under atmospheric conditions may be suitable for functionalizing arrays of CNTs, present on fiber or planar surfaces, used to prepare hierarchical composites9. However, the atmospheric plasma is more difficult to apply to dry powders and does not address the problems with agglomerates present in as-manufactured raw carbon nanomaterials.
In the present work, we introduce a detailed description of the ultrasonicated-ozonolysis (USO) method that we have previously applied to carbon nanomaterials12,13,14. The USO process is used to prepare stable, aqueous dispersions that are suitable for electrophoretically depositing (EPD) both CNTs and GNPs onto carbon and glass fibers. Examples of EPD using USO-functionalized CNTs to deposit thin, uniform films onto stainless steel and carbon fabric substrates will be provided. Methods and typical results used to chemically characterize the functionalized CNTs and GNPs will also be provided, using both X-ray photoelectron spectroscopy (XPS) and Raman spectroscopy. A brief discussion of the characterization results in comparison with other functionalization techniques will be provided.
Work Health and Safety Notice
The effects of exposure to nanoparticles such as CNTs, on human health are not well understood. It is recommended that special measures be taken to minimize exposure to and avoid environmental contamination with CNT powders. Suggested hazard isolation measures include working within a HEPA filter-equipped fume cupboard and/or glove box. Occupational hygiene measures include wearing protective clothing and two layers of gloves and performing regular cleaning of surfaces using damp paper towels or a vacuum cleaner with a HEPA filter to remove stray CNT powders. Contaminated articles should be bagged for hazardous waste disposal.
Exposure to ozone can irritate the eyes, lungs, and respiratory system, and at higher concentrations may cause lung damage. It is recommended that measures be taken to minimize personal and environmental exposure to generated ozone gas. Isolation measures include working within a fume cupboard. As the return air stream will contain unused ozone, it should be passed through an ozone destruct unit before being releasing into the atmosphere. Dispersions that have had ozone bubbled through them will contain some dissolved ozone. After ozonolysis operations, allow the dispersions to sit for 1 h before undertaking further processing so that the ozone can undergo natural decomposition.

<table border="0" cellpadding="0" cellspacing="0" width="588">
	
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Ultrasonic bath</td>
			<td style="width:87px;">Soniclean</td>
			<td style="width:119px;">80TD</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Ultrasonic horn</td>
			<td style="width:87px;">Misonix</td>
			<td style="width:119px;">S-4000-010 with CL5 converter</td>
			<td>Daintree Scientific</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Flocell stainless steel water jacketed</td>
			<td style="width:87px;">Misonix</td>
			<td style="width:119px;">800BWJ</td>
			<td>Daintree Scientific</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Peristaltic pump</td>
			<td style="width:87px;">Masterflex easy-load</td>
			<td style="width:119px;">7518-00</td>
			<td></td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:216px;">Controller for peristaltic pump</td>
			<td style="width:87px;">Masterflex modular controller</td>
			<td style="width:119px;">7553-78</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Ozone generator</td>
			<td style="width:87px;">Ozone Solutions</td>
			<td style="width:119px;">TG-20</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Ozone destruct unit</td>
			<td style="width:87px;">Ozone Solutions</td>
			<td style="width:119px;">ODS-1</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Recirculating liquid cooler</td>
			<td style="width:87px;">Thermoline</td>
			<td style="width:119px;">TRC2-571-T</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Multi-mode power supply unit</td>
			<td style="width:87px;">TTi&#160;</td>
			<td style="width:119px;">EX752M</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">High resolution computing multimeter</td>
			<td style="width:87px;">TTi&#160;</td>
			<td style="width:119px;">1906</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">X-ray photoelectron spectroscopy</td>
			<td style="width:87px;">Kratos Analytical</td>
			<td style="width:119px;">Axis Nova</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">XPS analysis software</td>
			<td style="width:87px;">Casa Software</td>
			<td style="width:119px;">Casa XPS</td>
			<td>www.casaxps.com</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Kratos elemental library for use with Casa XPS</td>
			<td style="width:87px;">Casa Software</td>
			<td style="width:119px;">Download Kratos Related Files</td>
			<td>http://www.casaxps.com/kratos/</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Raman dispersive confocal microscope</td>
			<td style="width:87px;">Thermo</td>
			<td style="width:119px;">DXR</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Field emission scanning electron microscope</td>
			<td style="width:87px;">Leo</td>
			<td style="width:119px;">1530 VP</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Sputter coater with iridium target</td>
			<td style="width:87px;">Cressington</td>
			<td style="width:119px;">208 HR</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Thickness measurement unit</td>
			<td style="width:87px;">Cressington</td>
			<td style="width:119px;">mtm 20</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Magnetic stirrer</td>
			<td style="width:87px;">Stuart</td>
			<td style="width:119px;">CD162</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Analytical balance</td>
			<td style="width:87px;">Kern</td>
			<td style="width:119px;">ALS 220-4N</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Analytical balance</td>
			<td style="width:87px;">Mettler Toledo</td>
			<td style="width:119px;">NewClassic MF MS 2045</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Laboratory balance</td>
			<td style="width:87px;">Shimadzu</td>
			<td style="width:119px;">ELB 3000</td>
			<td></td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:216px;">Electrodes from 316 stainless steel sheet</td>
			<td style="width:87px;">RS Components</td>
			<td style="width:119px;">559-199</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Sanding sheets, P1000 grade</td>
			<td style="width:87px;">Norton</td>
			<td style="width:119px;">No-Fil A275</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Multi-walled carbon nanotubes</td>
			<td style="width:87px;">Hanwha</td>
			<td style="width:119px;">CM-95</td>
			<td>http://hcc.hanwha.co.kr/eng/business/bus_table/nano_02.jsp</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Graphene nanoplatelets</td>
			<td style="width:87px;">XG Sciences</td>
			<td style="width:119px;">XGNP Grade C</td>
			<td>http://xgsciences.com/products/graphene-nanoplatelets/grade-c/</td>
		</tr>
	</tbody>
</table>

functionalization and dispersion of carbon nanomaterials using an environmentally friendly ultrasonicated ozonolysis process

Functionalization of carbon nanomaterials is often a critical step that facilitates their integration into larger material systems and devices. In the as-received form, carbon nanomaterials, such as carbon nanotubes (CNTs) or graphene nanoplatelets (GNPs), may contain large agglomerates. Both agglomerates and impurities will diminish the benefits of the unique electrical and mechanical properties offered when CNTs or GNPs are incorporated into polymers or composite material systems. Whilst a variety of methods exist to functionalize carbon nanomaterials and to create stable dispersions, many the processes use harsh chemicals, organic solvents, or surfactants, which are environmentally unfriendly and may increase the processing burden when isolating the nanomaterials for subsequent use. The current research details the use of an alternative, environmentally friendly technique for functionalizing CNTs and GNPs. It produces stable, aqueous dispersions free of harmful chemicals. Both CNTs and GNPs can be added to water at concentrations up to 5 g/L and can be recirculated through a high-powered ultrasonic cell. The simultaneous injection of ozone into the cell progressively oxidizes the carbon nanomaterials, and the combined ultrasonication breaks down agglomerates and immediately exposes fresh material for functionalization. The prepared dispersions are ideally suited for the deposition of thin films onto solid substrates using electrophoretic deposition (EPD). CNTs and GNPs from the aqueous dispersions can be readily used to coat carbon- and glass-reinforcing fibers using EPD for the preparation of hierarchical composite materials.

Figure 3 shows the XPS wide-scan characterization of CNTs that had undergone USO treatment. CNTs that had not undergone USO show almost no oxygen content. As the USO time increases, the surface oxygen level increases. Figure 4 charts the oxygen-to-carbon ratio increases as a function of USO time. Table 1 shows the deconvoluted carbon species atomic concentrations of GNP treated with USO. The peak fitting used a combination of constrained ...

Watch this Scientific Journal Video about Functionalization and Dispersion of Carbon Nanomaterials Using an Environmentally Friendly Ultrasonicated Ozonolysis Process at JoVE.com

Functionalization and Dispersion of Carbon Nanomaterials Using an Environmentally Friendly Ultrasonicated Ozonolysis Process

Here, a novel method for the functionalization and stable dispersion of carbon nanomaterials in aqueous environments is described. Ozone is injected directly into an aqueous dispersion of carbon nanomaterial that is continuously recirculated through a high-powered ultrasonic cell.

Functionalization of carbon nanomaterials is often a critical step that facilitates their integration into larger material systems and devices. In the ...

The overall goal of this Ultrasonic Ozonolysis and Electrophoretic Deposition processes is to achieve a stable dispersion of carbon nanomaterials which can then be used for the preparation of hierarchichal composite materials. This method can answer key questions in the hierarchichal composites field. By providing a convenient method to controllably coat microfiber fabrics with carbon nanomaterials.The main advantage of this technique is that carbon nanomaterials such as nanotubes and nanoplatelets can be functionalized made compatible with polymeric resin systems without the use of harsh chemicals. To begin this procedure, transfer all needed materials into a glove box inside a hepa-filter equipped fume cupboard. Weigh the desired quantity of nanomaterials into a bottle and add water so that the final nanoparticle concentration is one gram per liter.Seal the bottle with a lid. Ultrasonicate for 30 minutes at 43 kilohertz and 60 watts to disperse the CNTs and GNPs. Next, carefully pour the nanoparticles suspension into a reactor flask containing a magnetic stir-bar.Assemble the experimental set-up as detailed in the text protocol. After this, insert the ozone bubbler and connect it to the ozone generator. Then, place the reactor flask into the refrigerated bath.Stir the solution to prevent the CNTs from settling out of the suspension. Turn on the cooling water recirculation unit to cool the ozone reactor and ultrasonic cell. Then, turn on the oxygen supply to the ozone generator.Adjust the flow rate to zero point five litters per minute. Turn on the ozone generator and allow it to run for 30 to 60 minutes. The use of a bubbler on the other side of the ozone destruct unit enables a quick check that the ozone generator is connected and flowing properly.After this, turn on the peristaltic pump and adjust it to zero point six seven hertz. Turn on the ultrasonic horn and adjust the power to 60 watts. Observe the sonication process for at least 30 minutes, to ensure the pumping and ultrasonic horn operation is stable.After the desired ultrasonicated ozonolysis processing time has elapsed, turn off the ozone generator, sonicator, and pump. Stir the carbon nanomaterial dispersion for one hour to allow the ozone and solution to decompose. To begin, sonicate the nanomaterial suspension if electrophoresis is not performed within 24 hours of the ultrasonicated ozonolysis processing.Then, prepare three stainless steel electrodes as outlined in the text protocol. Using P1000 aluminum oxide sandpaper abrade the electrodes. Clean the abraded electrodes in an ultrasonic cleaning bath for 10 minutes in ultra pure water.Place the electrodes in a clean oven to dry for 10 minutes at 100 degrees celsius. After this, move the eletrodes to a desicator to cool. When cooled, weigh the electrodes to be used for anotic deposition on a four figure analytical balance.Then assemble the electrodes as outlined in the text protocol. Transfer 35 milliliters of carbon nanomaterial dispersion to a 50 milliliter beaker. Secure the electrode fixture to a retort-stand.After this, slowly lower the electrodes to the bottom of the beaker. Attach the positive terminal of the DC power supply to the central anode. Next, attach the negative terminal to one of the outer cathodes.Using a lid with alligator clips, connect the two outer cathodes. With the power supply turned off, adjust the current to the maximum setting. Adjust the voltage to the required value for the desired study.Then, prepare a stopwatch and turn on the power supply for the required coding duration. After this, switch off the power supply. Slowly raise the electrode fixture out of the dispersion, ensuring that the film is not disturbed.Disconnect the terminals from the electrodes. Next, slowly rotate the electrode fixture to a horizontal orientation. Allow the film on the anode to dry evenly.After this, disassemble the fixture and dry the anode in an oven for one hour at 100 degrees celsius. Place the dried anode in the desicator to cool. Using an analytical scale, record the weight to four decimal places.Photograph the coded anode and repeat the coding and weighing process for each deposition time. Then, perform characterization and analysis as outlined in the text protocol. In this study, a novel method is used to functionalize and stably disperse carbon nanomaterials in an aqueous environment.X-ray photoelectron spectroscopy widescan characterization shows that CNTs that did not undergo USO treatment contain almost no oxygen. However, the surface oxygen level is seen to increase as the USO treatment time increases. A raman spectrum reveals the presence of defects in the CNTs which existed even before USO treatment.The G band is seen to shift from a wavenumber of 1, 576 to a wavenumber of 1, 582 with the second component becoming more pronounced at a wavenumber of 1, 618 as USO time increases. Noticeable differences are also seen in the intensity of the two D band at a wavenumber of 2, 698. And then the D and G band at a wavenumber of 2, 941 as USO time increases.These changes all correspond with an increase in the oxidation level of the CNTs. Scanning electron microscopy shows that while untreated CNTs are highly compt, CNTs treated with USO are much more evenly distributed across the surface. This indicates that USO treatments has helped to reduce agglomirates present in the as received material.The USO treated CNTs are then electrophorically deposited using a variety of conditions, thicker films are produced as electrophoresis time increases. Thicker films are also produced when higher CNT concentrations are used. After its development, this technique paved the way for researchers in the field of hierarchical composites to explore whether Ultrasonic Ozonolysis can be used to functionalize other nanomaterials for eletrophoretic deposition.Different coating levels of carbon nanomaterials can have a significant effect on the macroscale composite properties such as strength and toughness. Because the method is water based after funtionalization, the ozone breaks down into harmless oxygen. And there is no need to remove chemicals from the dispersion before using the nanomaterials.Don't forget to run the ozone for one hour before turning on the peristaltic pump. Otherwise, you'll get clumping of nanomaterials in the tube.

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10.1K ビュー.  Defence Science and Technology Group. The overall goal of this Ultrasonic Ozonolysis and Electrophoretic Deposition processes is to achieve a stable dispersion of carbon nanomaterials which can then be used for the preparation of hierarchichal composite materials. This method can answer key questions in the hierarchichal composites field. By providing a convenient method to controllably coat microfiber fabrics with carbon nanomaterials.The main advantage of this technique is that carbon nanomaterials such as nanotubes and ...