Name: effects of exposure of formaldehyde to a rat model of atopic dermatitis induced by neonatal capsaicin treatment
Uploaded: 2017-09-27T12:30:00.000+00:00
Duration: 6 min 47 s
Description: Watch this Scientific Journal Video about Effects of Exposure of Formaldehyde to a Rat Model of Atopic Dermatitis Induced by Neonatal Capsaicin Treatment at JoVE.com

本研究は、公共の福祉によって支えられた &#38; 安全研究プログラムを通じて、国立研究財団の韓国 (NRF) 文部省、科学および技術 (NRF 2014M3C8A5030612)、によって資金を供給、鄭陽、チャチャ ヤングによってサポートされて太陽、MD やチャンこんにちはチュ ユー Sa 記念基金します。

ここで説明したすべてのメソッドが出生後 6 週間国際動物ケアおよび使用委員会 (IACUC) の韓国大学大学の医学によって承認されている、実験者ができる enthansasia を誘導すると収集。皮膚、ラットの血清など様々 な試料。
 1 新生児カプサイシン処置によって広告の誘導 <ol><li>カプサイシン ソリューション 5 mg/mL を準備するには、トゥイーン 80、15 mL の円錐管の 100% エタノール 1 mL に 1 mL に 50 mg のカプサイシンを追加します。</li>。
	<li>は、円錐管に生理食塩水入りの 8 mL を追加します。表示しているレイヤー分離がなくなったまで精力的にチューブを振る</li> <li>正中線と新生児 Sprague-dawley ラットの首の裏側に、皮下組織にカプサイシン溶液 (10 μ L/g) を注入します。
	<ol><li>配信 (重大) 生後後 48 時間 12 h 間カプサイシン 。
		メモ: 注入がより数日遅れた場合皮膚炎は開発しません、それは数週間のためにのみ保持されます。注入が早すぎる場合、呼吸が激しい痛みのため発生する可能性が</li>。
		カプサイシン投与による呼吸停止から新生児ラットを防ぐために <li>を押して呼吸を助長するまたは呼吸になるノーマル。 以来、カプサイシン誘発の痛みすることができますまで酸素室おいて、新生児に新生児の胸部呼吸の阻止、おそらく呼吸困難を悪化させる全身麻酔を追加します。実験者がラットの生存を増加する anesthesics を使用しないでくださいしたがって、.</li>
	</ol></li></ol> 2。掻痒症と広告のモデルラットにおける皮膚炎の評価 <ol><li><ol><li>準備プラスチックの 自発の掻破行動を数えることによってそう痒症の測定 室 (20 cm × 30 cm × 20 cm) 完全なカバレッジを許可するミラーを装備閲覧と呼吸するラットを許可するように穴にします</li>。
		<li>設定して正しくミラー ビューおよびラットのフロント ビューを同時に記録するデジタル ビデオ カメラを調整します</li>。
		<li>商工会議所や開口部を防ぐために重いオブジェクトの部屋の天井をカバー プラスチック場所ラット</li>。
		<li>1 h のラットの自発行動を記録、ビデオ クリップを再生および動作の傷の数をカウントします</li>。
	</ol></li> <li>得点システムを使用して皮膚病変の評価 <ol><li>誘導室でラットを配置します</li>。
		<li>2 L/分の酸素流量計をオンに流量し、麻酔の 3% にイソフルラン気化器を調整します</li>。
		<li>程度とラットの他の部分と耳の皮膚炎の重症度を識別します</li>。
		<li>0.25 cm 2 と次 の表 1 による皮膚病変の重症度として皮膚病変の範囲の単位を定義する: 重症度; 度程度と重症度を乗じて皮膚炎スコア (皮膚炎のスコアを参照してください <強いクラス ="xfig"> 図 1B).</li>
	</ol></li></ol> 3。ガス FA の動物への暴露 <ol><li>吸入室の準備 <ol><li>アクリル ガラス ボックスを準備 (55 cm × 40 cm × 35 cm) 反対側に 2 つのアウトレットがある</li>。
		<li>注意。FA ガスの循環、入口より出口を配置し、入口よりも出口のサイズを大ききます</li>。
		<li>注意。、店舗別の側面の内部のガス濃度測定の閉じることができます 3 つの小さな通路をドリルします。</li>
		両方の店に <li>接続チューブ。循環フードまたはガスの放出のために建物の外排水チューブを置き、他のアウトレット チューブを医療用酸素レギュレータに接続します</li>。
		<li>アクリル ガラス ボックス内に従来のラットのおりを配置します</li>。
		<li>(2 時間/日、5 日/週) 5 週間出産後 1 週間からネズミをケージに入れています</li>。
		<li>シール アクリル ガラス ボックス ガス漏れを防ぐために可能な限り緊密 。
		注: タイトなシールの室蓋はゴムを含める必要があります。密封のために部屋をラップするラップを適用します</li>。
	</ol></li> <li>医療用酸素レギュレータを用いた気体 FA の世代 <ol><li>FA の 5 g を 1 L の水に溶解する</li>。
		<li>医療用酸素レギュレータの加湿器ボトルに入れ、0.5 %fa ソリューションです</li>。
		<li>レギュレータにインレット チューブを接続した後、レギュレータをオンにし 5 L/分の流量を設定</li>
		<li>溶液濃度とチャンバー内のガスの濃度を決定するための流量を調整します</li>。
	</ol>
	</li>
</ol>

<ol>
	<li>Geha, R. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Allergy+and+hypersensitivity.+Nature+versus+nurture+in+allergy+and+hypersensitivity.">Allergy and hypersensitivity. Nature versus nurture in allergy and hypersensitivity.</a> Curr Opin Immunol. 15 (6), 603-608 (2003).</li><li>Novak, N., Bieber, T., Leung, D. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Immune+mechanisms+leading+to+atopic+dermatitis.">Immune mechanisms leading to atopic dermatitis.</a> The J Allergy Clin Immunol. 112 (Suppl 6), S128-S139 (2003).</li><li>Back, S. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chronically+relapsing+pruritic+dermatitis+in+the+rats+treated+as+neonate+with+capsaicin;+a+potential+rat+model+of+human+atopic+dermatitis.">Chronically relapsing pruritic dermatitis in the rats treated as neonate with capsaicin; a potential rat model of human atopic dermatitis.</a> J Dermatol Sci. 67 (2), 111-119 (2012).</li><li>Jin, H., He, R., Oyoshi, M., Geha, R. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+models+of+atopic+dermatitis.">Animal models of atopic dermatitis.</a> J Invest Dermatol. 129 (1), 31-40 (2009).</li><li>Han, R. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Formaldehyde-Induced+Aggravation+of+Pruritus+and+Dermatitis+Is+Associated+with+the+Elevated+Expression+of+Th1+Cytokines+in+a+Rat+Model+of+Atopic+Dermatitis.">Formaldehyde-Induced Aggravation of Pruritus and Dermatitis Is Associated with the Elevated Expression of Th1 Cytokines in a Rat Model of Atopic Dermatitis.</a> PLOS ONE. 11 (12), e0168466 (2016).</li><li>Xu, B., Aoyama, K., Takeuchi, M., Matsushita, T., Takeuchi, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Expression+of+cytokine+mRNAs+in+mice+cutaneously+exposed+to+formaldehyde.">Expression of cytokine mRNAs in mice cutaneously exposed to formaldehyde.</a> Immunol Lett. 84 (1), 49-55 (2002).</li><li>Kim, J. Y., Jeong, M. S., Park, K. Y., Seo, S. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aggravation+of+atopic+dermatitis-like+symptoms+by+consecutive+low+concentration+of+formaldehyde+exposure+in+NC/Nga+mice.">Aggravation of atopic dermatitis-like symptoms by consecutive low concentration of formaldehyde exposure in NC/Nga mice.</a> Exp Dermatol. 22 (3), 219-221 (2013).</li><li>Laske, N., Niggemann, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Does+the+severity+of+atopic+dermatitis+correlate+with+serum+IgE+levels?.">Does the severity of atopic dermatitis correlate with serum IgE levels?.</a> Pediatr Allergy Immunol. 15 (1), 86-88 (2004).</li><li>Fujii, Y., Takeuchi, H., Sakuma, S., Sengoku, T., Takakura, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+a+2,4-dinitrochlorobenzene-induced+chronic+dermatitis+model+in+rats.">Characterization of a 2,4-dinitrochlorobenzene-induced chronic dermatitis model in rats.</a> Skin Pharmacol Physiol. 22 (5), 240-247 (2009).</li><li>Sugimoto, T., Xiao, C., Ichikawa, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neonatal+primary+neuronal+death+induced+by+capsaicin+and+axotomy+involves+an+apoptotic+mechanism.">Neonatal primary neuronal death induced by capsaicin and axotomy involves an apoptotic mechanism.</a> Brain Res. 807 (1-2), 147-154 (1998).</li><li>Saito, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+skin+inflammation+induced+by+repeated+exposure+of+toluene,+xylene,+and+formaldehyde+in+mice.">Characterization of skin inflammation induced by repeated exposure of toluene, xylene, and formaldehyde in mice.</a> Environ Toxicol. 26 (3), 224-232 (2011).</li><li>Jung, W. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Formaldehyde+exposure+induces+airway+inflammation+by+increasing+eosinophil+infiltrations+through+the+regulation+of+reactive+oxygen+species+production.">Formaldehyde exposure induces airway inflammation by increasing eosinophil infiltrations through the regulation of reactive oxygen species production.</a> Environ Toixcol Pharmacol. 24 (2), 174-182 (2007).</li></ol>

著者が明らかに何もありません。

ラットの広告のような症状の誘発のためここで説明されたプロトコルは、AD の病態を研究するために適応し、薬物検査のスクリーニング ツールとして使用できます。ガス FA のカプサイシン投与ラットへの暴露の楽器は、多様な動物モデルに及ぼす各種ガスへの暴露の評価に適用できます。
新生児カプサイシン処理広告モデル、DNCB 治療モデル9と皮膚感...

ホルムアルデヒド (FA) アトピー性皮膚炎 (AD) の進行の効果を調査するには、広告誘導プロトコルを設立し、FA の慢性露出のための従来の循環室を開発しました。
広告は、遺伝的素因と環境トリガー1,2間の複雑な相互作用に起因する慢性の炎症性皮膚疾患です。適切な動物モデルは臨床状態で広告を勉強する必要があります。これは、環境要因の影響の下で AD の病因を解明するとき最も重要です。また、広告進行に及ぼす環境的誘因をよく調べるためには、それは個々 の環境要因を分離し、暴露の程度を定量化することが欠かせません。
我々 は新生児カプサイシン処置による広告の新規ラットモデルを実証しました。この広告モデル3皮膚感作性, 遺伝子組み換え広告モデルおよび広告4の自発的なマウス ・ モデルによる広告モデルなど他の広告モデルにいくつかのメリットがあります。まず、このモデルは幼児広告に対応し、Nc/nga マウスなど広告の他の自発的なマウス モデルより前は 3 週間の時代に広告のような症状を開発しています。遺伝子組み換えの広告モデルとは異なり、いくつかのケースで広告モデルの症状は治まるし、自発的に 20 週間にわたって再発します。また、モデルの広告のような症状の誘導プロセスはより誘導プロセスの観点からアレルギー性の接触皮膚炎に似ている皮膚感作モデルより簡単です。
小児および思春期の期間に広告の開発の FA 吸入の効果を研究するアクリル ガラス室と従来の医療用酸素レギュレータ5を使用して FA 曝露動物モデルにおける新しいプロトコルを紹介しています。レギュレータを一定濃度ガス流量を調整することによって、溶液濃度に動物を公開できました。以前の研究は、動物モデルへの FA の暴露のいくつかの方法を報告しています。徐らは、どの FA ソリューション皮膚6へ適用した直接露光法を説明します。別の最近の研究は対処モデル 49127特殊揮発性溶剤の発電機を用いた FA ガス曝露プロトコルです。従来の方法と比較して、新しい方法がより簡単に装備されてし、FA に加えて他のソリューションの適用することができます。

<table><tbody><tr><td>Medical Oxygen Regulator</td><td>Yuyao Jiahua Medical</td><td></td><td>manufacturer will not affect results</td></tr><tr><td>Tube</td><td></td><td></td><td>manufacturer will not affect results</td></tr><tr><td>Acrylic Chamber</td><td>Taejin Acryl</td><td></td><td>manufacturer will not affect results</td></tr><tr><td>Plastic Chamber</td><td>Taejin Acryl</td><td></td><td>manufacturer will not affect results</td></tr><tr><td>Paraformaldehyde, powder</td><td>Sigma</td><td>158127</td><td>manufacturer will not affect results</td></tr><tr><td>Wrap filme</td><td>Uniwrap</td><td></td><td>manufacturer will not affect results</td></tr><tr><td>Capsaicin</td><td>Sigma</td><td>M2028</td><td>manufacturer will not affect results</td></tr><tr><td>Tween 80</td><td>Sigma</td><td>P4780</td><td>manufacturer will not affect results</td></tr><tr><td>Digital Video Camera</td><td>Sony</td><td>HDR-CX380y</td><td>manufacturer will not affect results</td></tr><tr><td>Isoflurane</td><td>JW pharmaceutical</td><td></td><td>manufacturer will not affect results</td></tr></tbody></table>

effects of exposure of formaldehyde to a rat model of atopic dermatitis induced by neonatal capsaicin treatment

アトピー性皮膚炎掻痒湿疹を慢性的な再発は、先進国を中心に世界中で勝ちます。遺伝・環境要因間の複雑な相互作用は、アトピー性皮膚炎の病態に重要な役割を再生する知られています。しかし、我々 はまだこの病気の病因の詳細な画像を欠いています。したがって、アトピー性皮膚炎の進行を解明するための適切な動物モデルの開発に重要なのです。また、アトピー性皮膚炎に及ぼす大気汚染物質などの環境要因を調査して病気の理解を広げます。ここでは、新生児カプサイシン処置ラットにおけるアトピー性皮膚炎とアトピー性皮膚炎小児と思春期の発達に及ぼす影響を明らかにするラットへの一定ホルムアルデヒド濃度の暴露のためのプロトコルを誘導する方法を述べる期間。これらのプロトコルはいくつかの実験に正常に適用されているし、他の物質に使用することができます。

7 週齢でカプサイシン処理広告ラット モデルの広告のような皮膚の炎症は、図 1 aに提示されます。広告ラットのカプサイシン処理モデルの堅牢な掻破行動をビデオ 1に示します。新生児カプサイシン処置が皮膚炎を誘発 3 週齢ラットの行動を傷を悪化し、広告のような症状が数週間 (図 1 bC) の永続...

Watch this Scientific Journal Video about Effects of Exposure of Formaldehyde to a Rat Model of Atopic Dermatitis Induced by Neonatal Capsaicin Treatment at JoVE.com

Effects of Exposure of Formaldehyde to a Rat Model of Atopic Dermatitis Induced by Neonatal Capsaicin Treatment

新生児カプサイシン処置ラットとアトピー性皮膚炎に対するホルムアルデヒド吸入の影響を調査するため気化したホルムアルデヒドの露出でアトピー性皮膚炎を誘発する方法をご紹介します。

新生児カプサイシン処置によるアトピー性皮膚炎のモデルラットにホルムアルデヒドの暴露の影響

Atopic dermatitis is chronically relapsing pruritic eczema and prevails around the world especially in developed countries. Complex interactions between ...

effects-exposure-formaldehyde-to-rat-model-atopic-dermatitis-induced

Research

JoVE Journal

Immunology and Infection

9.6K ビュー.  Korea University. 新生児カプサイシン処置ラットとアトピー性皮膚炎に対するホルムアルデヒド吸入の影響を調査するため気化したホルムアルデヒドの露出でアトピー性皮膚炎を誘発する方法をご紹介します。

ビデオ: Effects of Exposure of Formaldehyde to a Rat Model of Atopic Dermatitis Induced by Neonatal Capsaicin Treatment

Chronic Post-Ischemia Pain Model for Complex Regional Pain Syndrome Type-I in Rats

Complex regional pain syndrome type-I (CRPS-I) is a neurological disease that causes severe pain among patients and remains an unresolved medical condition. However, the underlying mechanisms of CRPS-I have yet to be revealed. It is known that ischemia/reperfusion is one of the leading factors that causes CRPS-I. By means of prolonged ischemia and reperfusion of the hind limb, the rat chronic post-ischemia pain (CPIP) model has been established to mimic CRPS-I. The CPIP model has become a well-recognized animal model for studying the mechanisms of CRPS-I. This protocol describes the detailed procedures involved in the establishment of the rat model of CPIP, including anesthesia, followed by ischemia/reperfusion of the hind limb. Characteristics of the rat CPIP model are further evaluated by measuring the mechanical and thermal hypersensitivities of the hind limb as well as the nocifensive responses to acute capsaicin injection. The rat CPIP model exhibits several CRPS-I-like manifestations, including hind limb edema and hyperemia in the early stage after establishment, persistent thermal and mechanical hypersensitivities, and increased nocifensive responses to acute capsaicin injection. These characteristics render it a suitable animal model for further investigation of the mechanisms involved in CRPS-I.

Provided here is a protocol that details steps to establish an animal model of chronic post-ischemia pain (CPIP). This is a well-recognized model mimicking human complex regional pain syndrome type-I. Mechanical and thermal hypersensitivities are further evaluated, as well as capsaicin-induced nocifensive behaviors observed in the CPIP rat model.

ラットにおける複雑な局所疼痛症候群タイプIに対する慢性虚血後疼痛モデル

Complex regional pain syndrome type-I (CRPS-I) is a neurological disease that causes severe pain among patients and remains an unresolved medical ...

行動学

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Easy-to-use and inexpensive techniques are needed to determine the site-specific production of inflammatory mediators and neurotrophins during skin injury, inflammation, and/or sensitization. The goal of this study is to describe an epidermal-dermal separation protocol using thermolysin, a proteinase that is active at 4 &deg;C. To illustrate this procedure, Sprague Dawley rats are anesthetized, and right hind paws are injected with carrageenan. Six and twelve hours after injection, rats with inflammation and na&iuml;ve rats are euthanized, and a piece of hind paw, glabrous skin is placed in cold Dulbecco's Modified Eagle Medium. The epidermis is then separated at the basement membrane from the dermis by thermolysin in PBS with calcium chloride. Next, the dermis is secured by microdissection forceps, and the epidermis is gently teased away. Toluidine blue staining of tissue sections show that the epidermis is separated cleanly from the dermis at the basement membrane. All keratinocyte cell layers remain intact, and the epidermal rete ridges along with indentations from dermal papillae are clearly observed. Qualitative and real-time RT-PCR is used to determine nerve growth factor and interleukin-6 expression levels. Western blotting and immunohistochemistry are finally performed to detect amounts of nerve growth factor. This report illustrates that cold thermolysin digestion is an effective method to separate epidermis from dermis for evaluation of mRNA and protein alterations during inflammation.

Presented here is a protocol for the separation of epidermis from dermis to evaluate inflammatory mediator production. Following inflammation, rat hind paw epidermis is separated from the dermis by thermolysin at 4 &#176;C. The epidermis is then used for mRNA analysis by RT-PCR and protein evaluation by western blot and immunohistochemistry.

ラット表皮と真皮をサーモリジンと分離して、部位特異的炎症性mRNAおよびタンパク質を検出する

Easy-to-use and inexpensive techniques are needed to determine the site-specific production of inflammatory mediators and neurotrophins during skin ...

免疫・感染学

Cheek Injection Model for Simultaneous Measurement of Pain and Itch-related Behaviors

Itch was defined as &#34;an unpleasant cutaneous sensation that provokes a desire to scratch&#34;&#160;by Rothman in 1941. In mouse models, scratch bouts are typically counted to evaluate itch induced by pruritogens. However, previous reports have shown that algesic substances also induce scratching behaviors in a mouse neck injection model, which is the most common test used for scratching behaviors. This finding makes it difficult to study itch in mice. &#160;In contrast, capsaicin, a common algogen, reduced scratching behaviors in some neck injection experiments. Therefore, the effect of pain on scratching behaviors remains unclear. It is thus necessary to develop a method to concurrently investigate itch and pain sensation using behavioral tests. Here, a cheek injection model is introduced which can be used to simultaneously measure pain- and itch-related behaviors. In this model, pruritogens induce scratching behaviors while algesic substances induce wiping behaviors. Using this model, lysophosphatidic acid (LPA), an itch mediator found in cholestatic patients with itch, is shown to exclusively induce itch but not pain. However, in mouse models, LPA has been reported to be both a pruritogen and an algogen. Investigation into the effects of LPA in a mouse cheek injection model showed that LPA only induced scratching, but not wiping behaviors. This indicates that LPA acts as a pruritogen similarly in mice and humans, and demonstrates the utility of a cheek injection model for itch research.

Typically, the mouse neck injection model is used for evaluate pruritogen-induced scratch behaviors. However, the model provides information only on itch, not pain. Here, a cheek injection model is introduced in mice which can be used to simultaneously measure pain and itch-related behaviors.

痛みとかゆみに関連する行動の同時測定のための頬注射モデル

Itch was defined as &#34;an unpleasant cutaneous sensation that provokes a desire to scratch&#34;&#160;by Rothman in 1941. In mouse models, scratch bouts ...

神経科学

Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis

Contact hypersensitivity (CHS) is an experimental model of allergic contact dermatitis (ACD) that can be studied in mice. This study aims to present an objective laboratory method that may help to study the CHS reaction in mice, which can be measured and quantified by various tests. To induce CHS, on day "0", mice were sensitized on a previously shaved spot by abdominal skin painting with the hapten 2,4,6-trinitrochlorobenzene (TNCB) in an acetone-ethanol mixture, whereas negative control mice were sham sensitized with vehicle alone-acetone-ethanol mixture. On day "4", the baseline ear thickness was measured with a micrometer prior to the elicitation of CHS (challenge) by painting both ears with diluted TNCB both in the test and control groups. After 24 h, the ear swelling was measured with a micrometer. CHS is an example of a T cell-mediated immune response that causes swelling in inflamed tissue, peaking 24 h after the skin challenge with the same hapten. An increase in ear edema correlated with augmented ear weight, myeloperoxidase (MPO) activity, pro-inflammatory cytokine concentration in the ear extracts, increased thickening of the edematous dermis in the histological examination, and ear vascular permeability. There was also an increase in the concentration of TNP-specific IgG1 antibodies in the sera of the test group when compared with the control mice. Additionally, CHS can be successfully transferred with the CHS-effector cells obtained from donors previously sensitized with TNCB. The CHS-effector cells were administered intravenously into na&#239;ve recipient mice, which were subsequently challenged with the same diluted hapten. Ear swelling was measured with a micrometer 24 h later.

Contact hypersensitivity (CHS) is a murine experimental model of allergic contact dermatitis (ACD). CHS is based on sensitization with reactive hapten by painting the shaved skin of the chest and abdomen, with a subsequent ear skin challenge with a diluted hapten, causing a swelling reaction that is assessed in various ways.

アレルギー性接触皮膚炎のマウスモデルとしての接触過敏症

Contact hypersensitivity (CHS) is an experimental model of allergic contact dermatitis (ACD) that can be studied in mice. This study aims to present an ...

A Mouse Ear Model for Allergic Contact Dermatitis Evaluation

Skin is the human body&#39;s first line of defense and one of the most exposed organs to environmental chemicals. Allergic contact dermatitis (ACD) is a common skin disease that manifests as a local rash, redness, and skin lesions. The occurrence and development of ACD are influenced by both genetic and environmental factors. Although many scholars have constructed a series of models of ACD in recent years, the experimental protocols of these models are all different, which makes it difficult for readers to establish them well. Therefore, a stable and efficient animal model is of great significance to further study the pathogenesis of atopic dermatitis. In this study, we detail a modeling method using 1-fluoro-2,4-dinitrobenzene (DNFB) to induce ACD-like symptoms in the ears of mice and describe several methods for assessing the severity of dermatitis during modeling. This experimental protocol has been successfully applied in some experiments and has a certain promotional role in the field of ACD research.

Here, we describe the methods for inducing allergic contact dermatitis in mouse ears by 1-fluoro-2,4-dinitrobenzene (DNFB) and how to evaluate the severity of allergic contact dermatitis.

アレルギー性接触皮膚炎評価のためのマウス耳モデル

Skin is the human body&#39;s first line of defense and one of the most exposed organs to environmental chemicals. Allergic contact dermatitis (ACD) is a ...

Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents

Calcitonin gene-related peptide (CGRP) was first discovered in the 1980s as a splice variant from the calcitonin gene. Since its discovery, its role in migraine pathophysiology has been well established, first by its potent vasodilator properties and subsequently by its presence and function as a neurotransmitter in the sensory trigeminovascular system. The migraine-provoking ability of CGRP gave support to the pharma industry to develop monoclonal antibodies and antagonists inhibiting the effect of CGRP. A new treatment paradigm has proven effective in the prophylactic treatment of migraine. One of the useful tools to further understand migraine mechanisms is the ex vivo model of CGRP release from the trigeminovascular system. It is a relatively simple method that can be used with various pharmacological tools to achieve know-how to further develop new effective migraine treatments. The present protocol describes a CGRP release model and the technique to quantify the effect of pharmacological agents on the amount of CGRP released from the trigeminovascular system in rodents. A procedure describing the experimental approach from euthanasia to the measurement of protein levels is provided. The essential isolation of the trigeminal ganglion and the trigeminal nucleus caudalis from both mice and rats and the preparation of rat dura mater are described in detail. Furthermore, representative results from both species (rats and mice) are presented. The technique is a key tool to investigate the molecular mechanisms involved in migraine pathophysiology by using various pharmacological compounds and genetically modified animals.

Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents

The present protocol describes the ex vivo calcitonin gene-related peptide (CGRP) release model and the strategy to quantify the effect of pharmacological agents on the amount of CGRP released from the trigeminovascular system in rodents.

エクスビボ げっ歯類の三叉神経血管系からのカルシトニン遺伝子関連ペプチドの放出

Calcitonin gene-related peptide (CGRP) was first discovered in the 1980s as a splice variant from the calcitonin gene. Since its discovery, its role in ...

<meta charset="utf8"></head><body>早期産後疼痛研究のための早産ラットモデルの作成

A Preterm Rat Model for Pain Studies

This research delves into the consequences of consistent pinprick stimulation on preterm offspring to ascertain its long-term implications for pain sensitivity. The primary objective of this protocol was to investigate the impact of neonatal pinprick stimuli on the pain threshold in the later stages of life using a preterm rat model. By establishing this model, we aim to advance the research on understanding and managing early postnatal pain associated with prematurity. The findings of this study indicate that while the baseline thresholds to mechanical stimuli remained unaffected, there was a notable increase in mechanical hypersensitivity following complete Freund's adjuvant (CFA) injection in adult rats. Interestingly, compared with male rats, female rats demonstrated heightened inflammatory hypersensitivity. Notably, maternal behavior, the weight of the litters, and the growth trajectory of the offspring remained unchanged by the stimulation. The manifestation of altered nociceptive responses in adulthood after neonatal painful stimuli could be indicative of changes in sensory processing and the functioning of glucocorticoid receptors. However, further research is needed to understand the underlying mechanisms involved and to develop interventions for the consequences of prematurity and neonatal pain in adults.

<meta charset="utf8"></head><body>疼痛研究のための早産ラットモデル

Here, we present a concise protocol for creating a preterm rat model, facilitating research on early postnatal pain management. The method involves performing a cesarean section three days before the expected birth, extracting preterm rat pups via hysterectomy, and integrating them with the biological offspring of a surrogate dam.

疼痛研究のための早産ラットモデル

This research delves into the consequences of consistent pinprick stimulation on preterm offspring to ascertain its long-term implications for pain ...

アレルギー性鼻炎ラットモデルにおける鼻組織の多色免疫蛍光法

Immunofluorescent Labeling in Nasal Mucosa Tissue Sections of Allergic Rhinitis Rats via Multicolor Immunoassay

Allergic rhinitis (AR) is a chronic, non-infectious inflammatory disease of the nasal mucosa, primarily mediated by specific immunoglobulin E (IgE), affecting approximately 10%-20% of the world's population. While immunofluorescence (IF) staining has long been a standard technique for detecting disease-specific protein expression, conventional IF techniques are limited in their ability to detect the expression levels of three or more proteins in the same sample. Consequently, multicolor IF techniques have been developed in recent years, which allow the simultaneous labeling of multiple targets in cells or tissues.
This protocol provides a comprehensive overview of the process for establishing a rat model of AR, obtaining nasal mucosal samples, and the technical procedures for multicolor immunofluorescence. All rats in the AR group exhibited typical symptoms such as sneezing, a runny nose, and an itchy nose, with behavioral observations scoring &#8805;5 points. Hematoxylin and eosin (H&amp;E) staining revealed increased inflammatory cell counts and disrupted nasal mucosal integrity in the AR group. Multicolor immunofluorescence (mIF) demonstrated increased expression of ROR&#947;t and TICAM-1, while Foxp3 expression decreased in the nasal mucosa tissue of AR rats.

著者スポットライト:多色免疫蛍光によるアレルギー性鼻炎研究の進歩

This protocol describes a multicolor immunofluorescence technique for evaluating the rat model of allergic rhinitis.

多色免疫測定法 による アレルギー性鼻炎ラットの鼻粘膜組織切片における免疫蛍光標識

Allergic rhinitis (AR) is a chronic, non-infectious inflammatory disease of the nasal mucosa, primarily mediated by specific immunoglobulin E (IgE), ...

新生児カプサイシン処置によるアトピー性皮膚炎のモデルラットにホルムアルデヒドの暴露の影響

要約

さらに動画を探す

この動画の章

ラットにおける複雑な局所疼痛症候群タイプIに対する慢性虚血後疼痛モデル

ラット表皮と真皮をサーモリジンと分離して、部位特異的炎症性mRNAおよびタンパク質を検出する

痛みとかゆみに関連する行動の同時測定のための頬注射モデル

アレルギー性接触皮膚炎のマウスモデルとしての接触過敏症

アレルギー性接触皮膚炎評価のためのマウス耳モデル

エクスビボげっ歯類の三叉神経血管系からのカルシトニン遺伝子関連ペプチドの放出

疼痛研究のための早産ラットモデル

疼痛研究のための早産ラットモデル

疼痛研究のための早産ラットモデル

多色免疫測定法によるアレルギー性鼻炎ラットの鼻粘膜組織切片における免疫蛍光標識

新生児カプサイシン処置によるアトピー性皮膚炎のモデルラットにホルムアルデヒドの暴露の影響

要約

さらに動画を探す

この動画の章

ラットにおける複雑な局所疼痛症候群タイプIに対する慢性虚血後疼痛モデル

ラット表皮と真皮をサーモリジンと分離して、部位特異的炎症性mRNAおよびタンパク質を検出する

痛みとかゆみに関連する行動の同時測定のための頬注射モデル

アレルギー性接触皮膚炎のマウスモデルとしての接触過敏症

アレルギー性接触皮膚炎評価のためのマウス耳モデル

エクスビボ げっ歯類の三叉神経血管系からのカルシトニン遺伝子関連ペプチドの放出

疼痛研究のための早産ラットモデル

疼痛研究のための早産ラットモデル

疼痛研究のための早産ラットモデル

多色免疫測定法 による アレルギー性鼻炎ラットの鼻粘膜組織切片における免疫蛍光標識

エクスビボげっ歯類の三叉神経血管系からのカルシトニン遺伝子関連ペプチドの放出

多色免疫測定法によるアレルギー性鼻炎ラットの鼻粘膜組織切片における免疫蛍光標識