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Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

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06:38 min

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October 12th, 2018

DOI :

10.3791/58413-v

October 12th, 2018

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18,367 Views

Kaya J.E. Matson¹, Anupama Sathyamurthy¹, Kory R. Johnson², Michael C. Kelly³^,⁴, Matthew W. Kelley³, Ariel J. Levine¹

¹Spinal Circuits and Plasticity Unit, National Institute of Neurological Disorders and Stroke, ²Bioinformatics Section, Information Technology Program, National Institute of Neurological Disorders and Stroke, ³Laboratory of Cochlear Development, National Institute on Deafness and Other Communication Disorders, ⁴Single Cell Analysis Facility, Frederick National Laboratory

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要約

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Single nucleus RNA Sequencing

Spinal Cord Nuclei Isolation

Central Nervous System

Tissue Dissociation

Frozen Tissue

Cell Type specific Changes

Disease Or Injury

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