Eiry Kobatake

Cellular biosensing system for discovery of protein synthesis inhibitors with an electrochemical phosphate modulator to regulate the acid phosphatase gene expression of Saccharomyces cerevisiae.

Direct atomic force microscopy visualization of integration host factor-induced DNA bending structure of the promoter regulatory region on the Pseudomonas TOL plasmid.

Non-destructive monitoring of rpoS promoter activity as stress marker for evaluating cellular physiological status.

Ribosome display for selection of active dihydrofolate reductase mutants using immobilized methotrexate on agarose beads.

Atomic force microscopy identification of transcription factor NFkappaB bound to streptavidin-pin-holding DNA probe.

Induction of neural differentiation by electrically stimulated gene expression of NeuroD2.

A functional study on polymorphism of the ATP-binding cassette transporter ABCG2: critical role of arginine-482 in methotrexate transport.

The secretory response through electric stimulation of differentiated PC12 rat pheochromocytoma cells transfected with neuropeptide Y fused with enhanced green fluorescent protein.

Cellular biosensing system for assessing immunomodulating effects on the inducible nitric oxide synthase (iNOS) cascade.

Development of immune cellular biosensing system for assessing chemicals on inducible nitric oxide synthase signaling activator.

Intracellular delivery of antibodies using TAT fusion protein A.

Design and construction of glutamine binding proteins with a self-adhering capability to unmodified hydrophobic surfaces as reagentless fluorescence sensing devices.

The construction of endothelial cellular biosensing system for the control of blood pressure drugs.

Combined effect of electrical stimulation and cisplatin in HeLa cell death.

On-chip biosensing of estrogen receptor-alpha at single molecular level.

Construction of streptavidin-luciferase fusion protein for ATP sensing with fixed form.

Translational control by internal ribosome entry site in Saccharomyces cerevisiae.

Electrochemical evaluation of cellular physiological status under stress in Escherichia coli with the rpoS-lacZ reporter gene.

Method for detection of specific nucleic acids by recombinant protein with fluorescent resonance energy transfer.

Activity-based in vitro selection of T4 DNA ligase.

Glucose oxidase assisted homogeneous electrochemical receptor binding assay for drug screening.

Fabrication of an antibody microwell array with self-adhering antibody binding protein.

Construction of epidermal growth factor fusion protein with cell adhesive activity.

Identification of cysteine residues critically involved in homodimer formation and protein expression of human ATP-binding cassette transporter ABCG2: a new approach using the flp recombinase system.

Cell-surface-localized ATP detection with immobilized firefly luciferase.

In vitro selection of zinc finger DNA-binding proteins through ribosome display.

Transduction of NeuroD2 protein induced neural cell differentiation.

Delivery of antibody-captured proteins into living cells using PTD-fused protein A.

IgG binding kinetics to oligo B protein A domains on lipid layers immobilized on a 27 MHz quartz-crystal microbalance.

Assessment of small ligand-protein interactions by electrophoretic mobility shift assay using DNA-modified ligand as a sensing probe.

Novel recombination system using Cre recombinase alpha complementation.

Design of a thermocontrollable protein complex.

Direct detection of RNA transcription by FRET imaging using fluorescent protein probe.

Novel extracellular matrix for cell sheet recovery using genetically engineered elastin-like protein.

Construction of multi-functional extracellular matrix proteins that promote tube formation of endothelial cells.

Selection of mRNA 5'-untranslated region sequence with high translation efficiency through ribosome display.

RNA detection using peptide-inserted Renilla luciferase.

Construction of multifunctional proteins for tissue engineering: epidermal growth factor with collagen binding and cell adhesive activities.

Recombination system based on cre alpha complementation and leucine zipper fusions.

Transduction of MyoD protein into myoblasts induces myogenic differentiation without addition of protein transduction domain.

Construction of nanoscale protein particle using temperature-sensitive elastin-like peptide and polyaspartic acid chain.

Construction of a multi-functional extracellular matrix protein that increases number of N1E-115 neuroblast cells having neurites.

Promotion of angiogenesis by an artificial extracellular matrix protein containing the laminin-1-derived IKVAV sequence.

Evaluation of small ligand-protein interaction by ligation reaction with DNA-modified ligand.

Designed short peptides that form amyloid-like fibrils in coassembly with amyloid beta-peptide (Abeta) decrease the toxicity of Abeta to neuronal PC12 cells.

Evaluation of small ligand-protein interactions by using T7 RNA polymerase with DNA-modified ligand.

Targeted delivery using immunoliposomes with a lipid-modified antibody-binding protein.

Construction of a thermostable cell adhesion protein for reverse transfection.

Construction of a functional IgG-binding luciferase fusion protein for the rapid detection of specific bacterial strains.

Selection of DNA aptamers recognizing small cell lung cancer using living cell-SELEX.

Direct detection of RNAs in living cells using peptide-inserted Renilla luciferase.

Detection of small RNA molecules by a combination of branched rolling circle amplification and bioluminescent pyrophosphate assay.

Immuno-rolling circle amplification using a multibinding fusion protein.

Construction of a bFGF-tethered extracellular matrix using a coiled-coil helical interaction.

Homeostasis of extracellular ATP in human erythrocytes.

Cell fingerprint patterns using designed α-helical peptides to screen for cell-specific toxicity.

Construction of multi-functional extracellular matrix proteins that inhibits migration and tube formation of endothelial cells.

Development of a homogeneous immunoassay system using protein A fusion fragmented Renilla luciferase.

Construction of affinity changeable antibody in response to Ca2+.

Detection of antigens using a protein-DNA chimera developed by enzymatic covalent bonding with phiX gene A*.

Construction of semisynthetic DNA-protein conjugates with Phi X174 Gene-A* protein.

Development of a split SNAP-tag protein complementation assay for visualization of protein-protein interactions in living cells.

Aptamer-based protein detection using a bioluminescent fusion protein.

Induction of motor neuron differentiation by transduction of Olig2 protein.

Targeted gene delivery via PEI complexed with an antibody.

Genetic PEGylation.

Selection of DNA aptamers with affinity for pro-gastrin-releasing peptide (proGRP), a tumor marker for small cell lung cancer.

The promotion of angiogenesis by growth factors integrated with ECM proteins through coiled-coil structures.

Systematic screening of the cellular uptake of designed alpha-helix peptides.

Development of a specific siRNA delivery system into HeLa cells using an IgG-binding fusion protein.

In vitro selection of peptide aptamers using a ribosome display for a conducting polymer.

Construction of a bFGF-tethered multi-functional extracellular matrix protein through coiled-coil structures for neurite outgrowth induction.

Targeting of EGF-displayed protein nanoparticles with anticancer drugs.

Cellular differentiation assessments by measuring the degree of cellular internalization and membrane adsorption using designed peptides.

Assembly of zinc finger motif-fused enzymes on a dsDNA scaffold for catalyzing consecutive reactions with a proximity effect.

AFM characterization of chemically treated corneal cells.

Tracking a protein following dissociation from a protein-protein complex using a split SNAP-tag system.

Solubilization of single-walled carbon nanotubes using a peptide aptamer in water below the critical micelle concentration.

Growth Factor Tethering to Protein Nanoparticles via Coiled-Coil Formation for Targeted Drug Delivery.

An Engineered N-Cadherin Substrate for Differentiation, Survival, and Selection of Pluripotent Stem Cell-Derived Neural Progenitors.

Delivery of bFGF for Tissue Engineering by Tethering to the ECM.

Drug Delivery Using Novel Biological and Synthetic Materials.

Construction of a Defined Biomimetic Matrix for Long-Term Maintenance of Mouse Induced Pluripotent Stem Cells.

Development of a Split SNAP-CLIP Double Labeling System for Tracking Proteins Following Dissociation from Protein-Protein Complexes in Living Cells.

Design of luciferase-displaying protein nanoparticles for use as highly sensitive immunoassay detection probes.

Microfluidic-Nanofiber Hybrid Array for Screening of Cellular Microenvironments.

Fluorescent and luminescent fusion proteins for analyses of amyloid beta peptide aggregation.

Design of bFGF-tethered self-assembling extracellular matrix proteins via coiled-coil triple-helix formation.

Application of elastin-based nanoparticles displaying antibody binding domains for a homogeneous immunoassay.

A DNA-scaffold platform enhances a multi-enzymatic cycling reaction.