William E. Miller
Department of Molecular Genetics,
Biochemistry,
and Microbiology,
Department of Molecular Genetics, Biochemistry, and Microbiology
University of Cincinnati College of Medicine
William Miller is a Professor in the Department of Molecular Genetics, Biochemistry, and Microbiology at the University of Cincinnati College of Medicine. He earned his undergraduate degree in Genetics and Development from the Pennsylvania State University and obtained his Ph.D. in Microbiology and Immunology from the University of North Carolina at Chapel Hill under the guidance of Dr. Nancy Raab-Traub. Drs. Miller and Raab-Traub investigated the role of the Epstein-Barr Virus Latent Membrane Protein 1 in epithelial cell transformation and demonstrated that signaling through TNF receptor associated factors (TRAFs) played a central role in LMP1’s mechanisms of action.
After receiving his Ph.D. he pursued postdoctoral work with Dr. Robert J. Lefkowitz at Duke University in Durham, North Carolina where his work investigated mechanisms of G-protein Coupled Receptor (GPCR) Signaling and uncovered novel roles for the beta-arrestin proteins in GPCR signaling.
Dr. Miller joined the Department of Molecular Genetics, Biochemistry, and Microbiology in 2002 and established a laboratory focused on understanding the role of herpesvirus encoded GPCRs (vGPCRs) in promoting viral replication. His laboratory is currently focused on understanding how these vGPCRs facilitate replication in the salivary epithelium where it positions itself for efficient horizontal transmission. To that end, the laboratory has recently pursued the use of novel primary salivary cell systems to explore the roles of these vGPCRs within this tissue type.
G-protein-coupled receptor (GPCR) kinase phosphorylation and beta-arrestin recruitment regulate the constitutive signaling activity of the human cytomegalovirus US28 GPCR.
The Journal of biological chemistry Jun, 2003 | Pubmed ID: 12668664
beta-Arrestin inhibits NF-kappaB activity by means of its interaction with the NF-kappaB inhibitor IkappaBalpha.
Proceedings of the National Academy of Sciences of the United States of America Jun, 2004 | Pubmed ID: 15173580
A primate-dominant third glycosylation site of the beta2-adrenergic receptor routes receptors to degradation during agonist regulation.
The Journal of biological chemistry Sep, 2004 | Pubmed ID: 15247302
Signaling and regulation of G-protein coupled receptors encoded by cytomegaloviruses.
Biochemistry and cell biology = Biochimie et biologie cellulaire Dec, 2004 | Pubmed ID: 15674431
G protein-coupled receptor (GPCR) kinase 2 regulates agonist-independent Gq/11 signaling from the mouse cytomegalovirus GPCR M33.
The Journal of biological chemistry Dec, 2006 | Pubmed ID: 17088245
Pertussis toxin utilizes proximal components of the T-cell receptor complex to initiate signal transduction events in T cells.
Infection and immunity Aug, 2007 | Pubmed ID: 17562776
Desensitization of herpesvirus-encoded G protein-coupled receptors.
Life sciences Jan, 2008 | Pubmed ID: 18054964
Functional analysis of human cytomegalovirus pUS28 mutants in infected cells.
The Journal of general virology Jan, 2008 | Pubmed ID: 18089733
Beta-arrestin-dependent signaling and trafficking of 7-transmembrane receptors is reciprocally regulated by the deubiquitinase USP33 and the E3 ligase Mdm2.
Proceedings of the National Academy of Sciences of the United States of America Apr, 2009 | Pubmed ID: 19363159
Pertussis toxin signals through the TCR to initiate cross-desensitization of the chemokine receptor CXCR4.
Journal of immunology (Baltimore, Md. : 1950) May, 2009 | Pubmed ID: 19380820
Activation of intracellular signaling pathways by the murine cytomegalovirus G protein-coupled receptor M33 occurs via PLC-{beta}/PKC-dependent and -independent mechanisms.
Journal of virology Aug, 2009 | Pubmed ID: 19494016
The carboxy-terminal tail of human cytomegalovirus (HCMV) US28 regulates both chemokine-independent and chemokine-dependent signaling in HCMV-infected cells.
Journal of virology Oct, 2009 | Pubmed ID: 19605482
Mechanistic insight into pertussis toxin and lectin signaling using T cells engineered to express a CD8α/CD3ζ chimeric receptor.
Biochemistry May, 2012 | Pubmed ID: 22551306
US28 is a potent activator of phospholipase C during HCMV infection of clinically relevant target cells.
PloS one , 2012 | Pubmed ID: 23209769
Pertussis toxin B-pentamer mediates intercellular transfer of membrane proteins and lipids.
PloS one , 2013 | Pubmed ID: 24019885
β-Arrestin regulation of myosin light chain phosphorylation promotes AT1aR-mediated cell contraction and migration.
PloS one , 2013 | Pubmed ID: 24255721
The M33 G protein-coupled receptor encoded by murine cytomegalovirus is dispensable for hematogenous dissemination but is required for growth within the salivary gland.
Journal of virology Oct, 2014 | Pubmed ID: 25100846
The human cytomegalovirus lytic cycle is induced by 1,25-dihydroxyvitamin D3 in peripheral blood monocytes and in the THP-1 monocytic cell line.
Virology Sep, 2015 | Pubmed ID: 25965798
Cytomegalovirus Restructures Lipid Rafts via a US28/CDC42-Mediated Pathway, Enhancing Cholesterol Efflux from Host Cells.
Cell reports 06, 2016 | Pubmed ID: 27320924
The HCMV US28 vGPCR induces potent Gαq/PLC-β signaling in monocytes leading to increased adhesion to endothelial cells.
Virology 10, 2016 | Pubmed ID: 27497185
A little cooperation helps murine cytomegalovirus (MCMV) go a long way: MCMV co-infection rescues a chemokine salivary gland defect.
The Journal of general virology Nov, 2016 | Pubmed ID: 27638684
Epithelial Gpr116 regulates pulmonary alveolar homeostasis via Gq/11 signaling.
JCI insight Jun, 2017 | Pubmed ID: 28570277
Development of a Primary Human Cell Model for the Study of Human Cytomegalovirus Replication and Spread within Salivary Epithelium.
Journal of virology Feb, 2019 | Pubmed ID: 30404806
Human cytomegalovirus G protein-coupled receptor US28 promotes latency by attenuating c-fos.
Proceedings of the National Academy of Sciences of the United States of America 01, 2019 | Pubmed ID: 30647114
The Human Cytomegalovirus Chemokine vCXCL-1 Modulates Normal Dissemination Kinetics of Murine Cytomegalovirus .
mBio Jun, 2019 | Pubmed ID: 31239384
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