unfractionated-bulk-culture-mouse-skeletal-muscle-to-recapitulate

Unfractionated Bulk Culture of Mouse Skeletal Muscle to Recapitulate Niche and Stem Cell Quiescence

Skeletal muscle is the largest tissue of the body and performs multiple functions, from locomotion to body temperature control. Its functionality and ...

one-tissue-many-models-comprehensive-toolkit-for-skeletal-muscle

One Tissue, Many Models: Comprehensive Toolkit For Skeletal Muscle Studies

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Univ Paris Est Creteil, INSERM, IMRB, F-94010 Creteil, France

Univ Paris Est Creteil, INSERM, IMRB, F-94010 Creteil

Obesity triggers skeletal muscle physio-pathological alterations. However, the crosstalk between adipose tissue and myogenic cells remains poorly understood during obesity. We identified NID-1 among the adipose tissue secreted factors impairing myogenic potential of human myoblasts and murine muscle stem cells in vitro. Mice under High Fat Diet (HFD) displayed increased NID-1 expression in the skeletal muscle endomysium associated with intramuscular fat adipose tissue expansion and compromised muscle stem cell function. We show that NID-1 is highly secreted by skeletal muscle fibro-adipogenic/mesenchymal progenitors (FAPs) during obesity. We demonstrate that increased muscle NID-1 impairs muscle stem cells proliferation and primes the fibrogenic differentiation of FAPs, giving rise to an excessive deposition of extracellular matrix. Finally, we propose a model in which obesity leads to skeletal muscle extracellular matrix remodeling by FAPs, mediating the alteration of myogenic function by adipose tissue and highlighting the key role of NID-1 in the crosstalk between adipose tissue and skeletal muscle.

Matthew J. Borok

Univ Paris Est Creteil, INSERM, IMRB, F-94010 Creteil, France

Unfractionated Bulk Culture of Mouse Skeletal Muscle to Recapitulate Niche and Stem Cell Quiescence

One Tissue, Many Models: Comprehensive Toolkit For Skeletal Muscle Studies