Regulating Schwann Cell Growth by Nanosecond Pulsed Electric Field for Peripheral Nerve Regeneration In Vitro

593 Views

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09:24 min

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May 3rd, 2024

DOI :

10.3791/66097-v

May 3rd, 2024

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Jiahang Han*¹^,², Zewei Wang*¹^,², Yanzhao Dong*¹, Xiaodi Zou³, Haoyu Wang¹^,², Yonggang Chen¹, Sahar Ahmed Abdalbary⁴, Tian Tu⁵, Hui Lu¹

¹Department of Orthopedics, The First Affiliated Hospital, College of Medicine, Zhejiang University, ²College of Medicine, Zhejiang University, ³Department of Orthopedics, The Second Affiliated Hospital of Zhejiang Chinese Medical University, ⁴Department of Orthopedic Physical Therapy, Faculty of Physical Therapy, Nahda University in Beni Suef, ⁵Plastic & Cosmetic Center, The First Affiliated Hospital, College of Medicine, Zhejiang University

Transcript

Summary

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Schwann Cells

Peripheral Nerve Regeneration

Nanosecond Pulsed Electric Field

NsPEF

Cell Proliferation

Nerve Electrical Stimulation

Biological Processes

Neurotrophic Factors

Myelination Regulators

Chapters in this video

0:00

Introduction

1:27

Preparing Cryopreserved RSC96 Cells for Nanosecond Pulsed Electric Field Stimulation

3:36

Operation of the Nanosecond Pulsed Electric Field (nsPEF) Device to Stimulate Cultured RSC96 Cells

4:21

Validating the Cellular Proliferation and Migration of Nanosecond Pulsed Electric Field (nsPEF) Stimulated RSC96 Cells

6:28

Detecting the Expression of the Neurotrophic and Transcription Factors in Nanosecond Pulsed Electric Field (nsPEF) Stimulated RSC96 Cells

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