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In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures

DOI :

10.3791/63068-v

10:45 min

October 14th, 2021

October 14th, 2021

2,606 Views

1Laboratory of Axon Growth and Regeneration, Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)

This protocol demonstrates a straightforward and robust method to study in situ axon growth and growth cone dynamics. It describes how to prepare ex vivo physiologically relevant acute brain slices and provides a user-friendly analysis pipeline.

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Axon Growth

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