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University of Malaya

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Time-Lapse 2D Imaging of Phagocytic Activity in M1 Macrophage-4T1 Mouse Mammary Carcinoma Cells in Co-cultures

Noorzaileen Eileena Zaidi¹, Nur Aima Hafiza Shazali², Adam Leow Thean Chor¹, Mohd Azuraidi Osman¹, Kamariah Ibrahim³, Marilyn Jaoi-Edward⁴, Nik Mohd Afizan Nik Abd Rahman^1,2

¹Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, ²Institute of Tropical Forestry and Forestry Products (INTROP), Universiti Putra Malaysia, ³Department of Biomedical Science, Faculty of Medicine, University of Malaya, ⁴Agro-Biotechnology Institute (ABI), National Institute of Biotechnology Malaysia (NIBM)

Macrophage phagocytic activity against cancer cells, specifically 4T1 mouse mammary carcinoma cells, was imaged in this study. The live cell coculture model was established and observed using a combination of fluorescent and differential interference contrast microscopy. This assessment was imaged using imaging software to develop multipoint time-lapse video.

Medicine

Endoscopic Ultrasound-Guided Biliary Drainage: Endoscopic Ultrasound-Guided Hepaticogastrostomy in Malignant Biliary Obstruction

Stanley Khoo¹, Ida Hilmi¹, Jun Kit Koong², Peng Soon Koh², Boon Koon Yoong², Sanjiv Mahadeva¹

¹Gastroenterology and Hepatology Unit, Department of Medicine, Faulty of Medicine, University of Malaya, ²Hepato-Pancreato-Biliary Unit, Department of Surgery, Faculty of Medicine, University of Malaya

Endoscopic Ultrasound-Guided Biliary Drainage (EUS-BD) is an alternative method of biliary decompression in malignant biliary obstruction. Here we describe the technique of EUS guided-Hepaticogastrostomy (EUS-HGS) in a case of unresectable malignant hilar biliary obstruction.