Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological TestJulia Häring *1, Tanja Michel *1, Matthias Becker 1, Daniel Junker 1, Tatia Tchitchagua 2, Olaf Leschnik 2, Berit Lange 3,4, Stefanie Castell 3,4, Gérard Krause 3,4, Monika Strengert 3, Alex Dulovic 1, Nicole Schneiderhan-Marra 1
1NMI Natural and Medical Sciences Institute at the University of Tübingen, 2Department of Neurology, Sächsisches Krankenhaus Rodewisch, 3Department of Epidemiology, Helmholtz Centre for Infection Research, 4German Centre for Infection Research (DZIF)
A three-channel dual-reporter fluorescence flow analysis system was used to develop a bead-based multiplex immunoassay that simultaneously evaluates serum samples for IgG and IgM elicited against multiple antigens of different Borrelia species that cause Lyme borreliosis in Europe and North America.
