Simultaneous Isolation of Principal Central Nervous System-Resident Cell Types from Adult Autoimmune Encephalomyelitis MiceChristina B. Schroeter *1, Antonia Henes *1, Anna Vogelsang 1, Alexander M. Herrmann 1, Stefanie Lichtenberg 1, Derya Cengiz 1, Vera Dobelmann 1, Niklas Huntemann 1, Christopher Nelke 1, Susann Eichler 2, Philipp Albrecht 1, Sven G. Meuth *1, Tobias Ruck 1
1Department of Neurology, Medical Faculty, Heinrich Heine University Duesseldorf, 2Department of Neurology, Institute of Translational Neurology, University Hospital Muenster
To date, protocols for the simultaneous isolation of all principal central nervous system-resident cell types from the same mouse are an unmet demand. The protocol shows a procedure applicable in naïve and experimental autoimmune encephalomyelitis mice to investigate complex cellular networks during neuroinflammation and simultaneously reduce the required mice numbers.
