Stabilized Window for Intravital Imaging of the Murine PancreasJakeb Petersen *1,2,3, Wei Du *4,5, Christian Adkisson 1,4, Claudia Gravekamp 6,7, Maja H. Oktay 2,3,4,7,8,9, John Condeelis 1,3,4,7,8,9,10, Nicole C. Panarelli 2,3,7, John C. McAuliffe 1,2,3,7,8, David Entenberg 2,3,4,7,8,9
1Department of Surgery, Einstein College of Medicine / Montefiore Medical Center, 2Department of Pathology, Einstein College of Medicine / Montefiore Medical Center, 3Integrated Imaging Program for Cancer Research, Einstein College of Medicine / Montefiore Medical Center, 4Department of Anatomy and Structural Biology, Einstein College of Medicine / Montefiore Medical Center, 5Breast Center, Peking University People's Hospital, 6Department of Microbiology & Immunology, Einstein College of Medicine / Montefiore Medical Center, 7Montefiore Einstein Cancer Center, Einstein College of Medicine / Montefiore Medical Center, 8Cancer Dormancy and Tumor Microenvironment Institute, Albert Einstein College of Medicine / Montefiore Medical Center, 9Gruss-Lipper Biophotonics Center, Einstein College of Medicine / Montefiore Medical Center, 10Department of Cell Biology, Einstein College of Medicine / Montefiore Medical Center
We present a protocol for the surgical implantation of a stabilized indwelling optical window for subcellular-resolution imaging of the murine pancreas, allowing serial and longitudinal studies of the healthy and diseased pancreas.
