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German Cancer Research Center (DKFZ) Heidelberg

2 ARTICLES PUBLISHED IN JoVE

Medicine

Endotracheal Intubation via Tracheotomy and Subsequent Thoracotomy in Rats for Non-Survival Applications

Alexander Studier-Fischer^1,2,3,4, Berkin Özdemir^1,3,4, Karl-Friedrich Kowalewski^2,3,4, Caelan Max Haney^2,3,4,5, Maurice Stephan Michel^2,3,4, Gabriel Alexander Salg¹, Franck Billmann¹

¹Department of General, Visceral, and Transplantation Surgery, Heidelberg University Hospital, ²Department of Urology and Urosurgery, University Medical Center Mannheim, Medical Faculty of the University of Heidelberg, ³Intelligent Systems and Robotics in Urology (ISRU), German Cancer Research Center (DKFZ) Heidelberg, ⁴DKFZ Hector Cancer Institute, University Medical Center Mannheim, ⁵Department of Urology, University Hospital Leipzig

Here we introduce a standardized procedure for endotracheal intubation via tracheotomy followed by thoracotomy in rats, aimed at enhancing the precision and reproducibility of non-survival applications requiring invasive ventilation and exposure of thoracic organs in in vivo rat models.

Medicine

Controlled Reversible Visceral Arterial Ischemia, Venous Congestion and Combined Malperfusion via Midline Laparotomy in Rats

Alexander Studier-Fischer^1,2,3,4, Berkin Özdemir^1,3,4, Karl-Friedrich Kowalewski^2,3,4, Caelan Max Haney^2,3,4,5, Luisa Egen^2,3,4, Maurice Stephan Michel^2,3,4, Markus Mieth¹, Zoltan Czigany¹, Gabriel Alexander Salg¹, Henrik Nienhüser¹, Franck Billmann¹

¹Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, ²Department of Urology and Urosurgery, University Medical Center Mannheim, Medical Faculty of the University of Heidelberg, ³Division of Intelligent Systems and Robotics in Urology (ISRU), German Cancer Research Center (DKFZ) Heidelberg, ⁴DKFZ Hector Cancer Institute at the University Medical Center Mannheim, ⁵Department of Urology, University Hospital Leipzig

This article introduces a standardized procedure for controlled, reversible malperfusion of visceral organs in rat models. The aim is to induce these malperfusion states with a high degree of methodological certainty and control while maintaining technical simplicity and error resilience.