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Max Delbrück Center for Molecular Medicine (MDC)

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Generation and Downstream Analysis of Single-Cell and Single-Nuclei Transcriptomes in Brain Organoids

Miriam Wandres^*1,2, Denise Aigner^*1,2, Nicolai Kastelic^1,3, Anastasiya Boltengagen¹, Agnieszka Rybak-Wolf^1,3, Nikolaus Rajewsky^1,2

¹Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC), ²Institut für Biologie, Humboldt-Universität zu Berlin, ³Organoid platform, Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC)

Here, we introduce a comprehensive protocol for the generation and downstream analysis of human brain organoids using single-cell and single-nucleus RNA sequencing.