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Animal Epidemiology of the Ministry of Agriculture

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Examination of Pyroptosis by Flow Cytometry

He Chang^1,2,3, Zhi Chen^1,2,3, Li Gao^1,2,3, Hong Cao^1,2,3, Yongqiang Wang^1,2,3, Shijun J. Zheng^1,2,3

¹National Key Laboratory of Veterinary Public Health Security, ²Animal Epidemiology of the Ministry of Agriculture, ³College of Veterinary Medicine, China Agricultural University

This article describes the identification of pyroptotic cells using flow cytometry after dual staining with antibodies against the N-terminal fragment of chicken GSDME (chGSDME-^NT) and propidium iodide (PI).