Modified Langendorff Perfusion for Extended Perfusion Times of Rodent Cardiac GraftsCasie A. Pendexter 1,2, Maya Bolger-Chen 1,2, Manuela Lopera Higuita 1,2, Stephanie E. J. Cronin 1,2, S. Alireza Rabi 3, Asishana A. Osho 3, Shannon N. Tessier 1,2
1Center for Engineering in Medicine and Surgery, Harvard Medical School and Massachusetts General Hospital, 2Shriners Children's Boston, 3Division of Cardiac Surgery, Corrigan Minehan Heart Center, Massachusetts General Hospital
This article demonstrates the feasibility of achieving longer perfusion times (4 h) of murine cardiac grafts without function loss by employing lower (30-35 mmHg) than physiological (60-80 mmHg) perfusion pressures during Langendorff.
