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National Center for Respiratory Medicine

1 ARTICLES PUBLISHED IN JoVE

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Biology

In Vitro Model for Studying Differentiation and Changes of Multi-Omics on Murine Airway Epithelial Cells Stimulated with Cigarette Smoke Extract
Muzhi Zhang 1, Liuyinuo Zhao 1, Kaixin Li 1, Ruoyang Zhang 2,3, Zhe Lv 1, Jie Liu 1, Ye Cui 1, Wei Wang 1, Sun Ying 1
1Department of Immunology, School of Basic Medical Sciences, Capital Medical University, 2Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, China-Japan Friendship Hospital, 3National Center for Respiratory Medicine

Here, we describe an in vitro model for isolating and differentiating murine airway epithelial cells, focusing on their acclimation to chronic cigarette smoke extract (CSE). The model could be utilized to comprehensively characterize the multi-omics impact of CSE, which possibly provides insights into the cellular responses under chronic smoke exposure.

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