Long-term Monitoring of Oxygen Consumption Rates in Highly Differentiated and Polarized Retinal Pigment Epithelial CulturesQitao Zhang *1, Daisy Y. Shu *2, Richard A. Bryan 3, John Y. S. Han 1, Gillian A. Gulette 1, Kin Lo 3, Leo A. Kim 4, Jason M. L. Miller 1,5
1Kellogg Eye Center, University of Michigan, Ann Arbor, 2School of Optometry and Vision Science, University of New South Wales, 3Lucid Scientific, 4Schepens Eye Research Institute of Mass. Eye and Ear, Department of Ophthalmology, Harvard Medical School, 5Cellular and Molecular Biology Program, University of Michigan, Ann Arbor
We introduce a novel device for measuring oxygen consumption rates (OCR) in retinal pigment epithelial (RPE) cultures. The device can measure OCR for weeks at a time on RPE grown on standard cell culture plates with standard media while the plates are in a standard cell culture incubator.
