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Tufts Medical Center

2 ARTICLES PUBLISHED IN JoVE

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Biology

A 3D System for Culturing Human Articular Chondrocytes in Synovial Fluid
Joshua A. Brand 1, Timothy E. McAlindon 2, Li Zeng 1
1Department of Anatomy and Cellular Biology, Tufts University School of Medicine, 2Department of Rheumatology, Tufts Medical Center

A 3D system of culturing human articular chondrocytes in high levels of synovial fluid is described. Synovial fluid reflects the most natural microenvironment for articular cartilage, and can be easily obtained and stored. This system thus can be used for studying cartilage regeneration and for screening therapeutics for treating arthritis.

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Biology

Isolation of Mammary Epithelial Cells from Three-dimensional Mixed-cell Spheroid Co-culture
Kun Xu 1, Rachel J. Buchsbaum 1
1Molecular Oncology Research Institute, Department of Medicine, Tufts Medical Center

A simple method is described for analyzing effects of tissue fibroblasts on associated epithelial cells. The combination of this method and three-dimensional tissue culture can facilitate analysis of cells after isolation from 3D. The technique is applicable to cells of varying malignant potential, allowing systematic study of effects of tumor-associated stroma on tumor cells.

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