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University of Hawaiʻi at Mānoa

1 ARTICLES PUBLISHED IN JoVE

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Biology

Using Mycobacterium smegmatis as a Bioindicator for Zinc-Limited Growth Conditions in Mycobacteria
Allexa D. Burger *1, Jayna Wong *1, Endrei Marcantonio 1, Sladjana Prisic 1
1School of Life Sciences, University of Hawaiʻi at Mānoa

This protocol details preparation of Zn2+-limited media, growth vessels, and seed cultures to produce Zn2+-limited ribosomes in mycobacteria. Traces of Zn2+ lead to Zn2+-limiting growth and we describe morphogenic features used as bioindicators to verify Zn2+ limitation in vitro in the model bacterium, Mycobacterium smegmatis, and the human pathogen M. tuberculosis.

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