Fibro-Adipogenic Progenitor Isolation, Expansion, and Differentiation from the Spiny Mouse ModelBruce Lin 1, Hesham Soliman 1,2, Fabio M. V. Rossi 1, Marine Theret 1
1School of Biomedical Engineering and Department of Medical Genetics, University of British Columbia, 2Aspect Biosystems
This protocol outlines the isolation of skeletal and cardiac muscle fibro-adipogenic progenitors (FAPs) from spiny mouse (Acomys) via enzymatic dissociation and fluorescence-activated cell sorting. The FAPs obtained from this protocol can be effectively expanded and differentiated to myofibroblasts and adipocytes.
