In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in MouseHaofei Wang *1, Xu Liu *1, Wei Huang 1, Xiaojing Wu 1, Ying Tang 1, Mingzhu Zheng 1,2, Haibo Qiu 1, Yingzi Huang 1
1Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University, 2Department of Pathogenic Biology and Immunology, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine, Southeast University
This protocol describes the differentiation of naïve CD4+ T cells into pathogenic Th17 cells in vitro. Specifically, when combined with a multi-parameter flow cytometry-based approach, 90% purity of pathogenic Th17 cells can be obtained from naïve CD4+ T cells using this differentiation method.
