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Rush University

2 ARTICLES PUBLISHED IN JoVE

Biology

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Cristina Bardita^*1, Dan Predescu^*1,2, Sanda Predescu^1,2

¹Department of Pharmacology, Rush University, ²Pulmonary and Critical Care Department, Rush University

Repeated retro-orbital injections of cationic liposomes/siRNA/ITSN-1s complexes in mice, every 72 hours for 24 days, efficiently deliver the siRNA duplex to the mouse lung microvasculature reducing ITSN-1s mRNA and protein expression by 75%. This technique is highly reproducible in an animal model, has no adverse effects and avoids fatalities.

Biology

Neutron Radiography and Computed Tomography of Biological Systems at the Oak Ridge National Laboratory's High Flux Isotope Reactor

Hassina Z. Bilheux^*1, Maria Cekanova^*2,3,4, Jeffrey M. Warren^*5, Matthew J. Meagher⁶, Ryan D. Ross⁶, Jean C. Bilheux^1,7, Singanallur Venkatakrishnan⁸, Jiao Y.Y. Lin^1,9, Yuxuan Zhang¹, Matthew R. Pearson^9,10, Erik Stringfellow¹

¹Neutron Scattering Division, Oak Ridge National Laboratory, ²College of Veterinary Medicine, The University of Tennessee, ³UT-ORNL Graduate School of Genome, Science and Technology, The University of Tennessee, ⁴Integrity Laboratories, ⁵Environmental Sciences Division, Oak Ridge National Laboratory, ⁶Department of Cell & Molecular Medicine, Rush Medical College, Rush University, ⁷Computer Science and Mathematics Division, Oak Ridge National Laboratory, ⁸Electrification and Energy Infrastructures Division, Oak Ridge National Laboratory, ⁹Now at Second Target Station Project, Oak Ridge National Laboratory, ¹⁰Neutron Technologies Division, Oak Ridge National Laboratory

This manuscript describes a protocol for neutron radiography and computed tomography of biological samples using a High Flux Isotope Reactor (HFIR) CG-1D beamline to measure a metal implant in a rat femur, a mouse lung, and an herbaceous plant root/soil system.