Widespread Transduction of Mouse Neocortical Neurons by Subarachnoid Injection of AAV2Maria P. Smirnova 1, Aksiniya A. Osipova 1, Anastasia A. Borodinova 1, Tatyana M. Bagrinovtseva 1,2, Yulia V. Dobryakova 1, Ivan V. Smirnov 1, Alexey Y. Malyshev 1
1Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, 2Faculty of Biology and Biotechnology, National Research University High School of Economics
A new technique for widespread delivery of adeno-associated virus that uses subarachnoid virus infusion is described. This method not only ensures widespread transduction of mouse neocortical neurons in superficial layers but also results in selective expression of the transgene in layer five pyramidal neurons, even when using a non-selective promoter.
