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Laval University

7 ARTICLES PUBLISHED IN JoVE

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Medicine

Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining
Estelle Rousselet 1, Jasna Kriz 2, Nabil G. Seidah 1
1Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 2CHUQ Research Centre, Laval University

The intraluminal middle cerebral occlusion model in mice is herein presented. The extent of cerebral infarct is evaluated by a neurologic score and cresyl violet staining, an alternative staining to TTC, offering the great advantage to test in parallel many interest markers.

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Biology

Analysis of Translation Initiation During Stress Conditions by Polysome Profiling
Laëtitia Coudert *1,2, Pauline Adjibade *1,2, Rachid Mazroui 1,2
1Department of Molecular Biology, Medical Biochemistry, and Pathology, Faculty of Medicine, Laval University, 2CHU de Quebec Research Center

Here, we describe a method to analyze changes in the initiation of mRNA translation of eukaryotic cells in response to stress conditions. This method is based on the velocity separation on sucrose gradients of translating ribosomes from non-translating ribosomes.

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Developmental Biology

Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures
Julie Lessard 1, Julie Anne Côté 1, Marc Lapointe 1, Mélissa Pelletier 2, Mélanie Nadeau 1, Simon Marceau 1, Laurent Biertho 1, André Tchernof 1,2,3
1IUCPQ Research Center, 2CHU de Québec Research Center, 3Laval University

Mature adipocytes may represent an abundant source of stem cells through dedifferentiation, which leads to a homogenous population of fibroblast-like cells. Collagenase digestion is used to isolate mature adipocytes from human fat. The goal of our protocol is to obtain multipotent, dedifferentiated fat cells from human mature adipocytes.

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JoVE Journal

Increasing Pulmonary Artery Pulsatile Flow Improves Hypoxic Pulmonary Hypertension in Piglets
Audrey Courboulin 1, Chantal Kang 2, Olivier Baillard 3,4,5, Sebastien Bonnet 1, Pierre Bonnet 6
1Department of Medicine, Pulmonary Hypertension Research Group (CRIUCPQ), Laval University, 2Institut National de la Recherche Agronomique, 3Université Diderot Paris, Sorbonne Paris Cité, 4Hôpital Lariboisière, Physiologie clinique Explorations Fonctionnelles, 5INSERM U 965, 6Service de Cardiologie, Centre Hospitalier Universitaire Tours

Pulmonary hypertension is associated with a significant reduction in pulmonary artery pulsatility, contractility and elasticity, contributing to an increase in pulmonary artery pressure and pulmonary resistance. Using a hypoxic piglet model, this study demonstrated that improving pulmonary artery plasticity using a newly developed pulsatile catheter improves hypoxic pulmonary hypertension.

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Bioengineering

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
Sébastien Meghezi 1, Dawit G. Seifu 1,2, Nina Bono 1,3, Larry Unsworth 2,4,5, Kibret Mequanint 6, Diego Mantovani 1,2
1Laboratory for Biomaterials and Bioengineering, Department Min-Met-Materials Eng & CHU de Québec Research Center, Canada Research Chair I for the Innovation in Surgery, Laval University, 2NSERC CREATE Program for Regenerative Medicine (NCPRM), Laval University, 3Department Electronics, Information and Bioengineering, Politecnico di Milano, 4Department of Chemical and Materials Engineering, University of Alberta, 5National Institute for Nanotechnology, National Research Council (Canada), 6Department of Chemical and Biochemical Engineering, University of Western Ontario

In this work, we present a technique for the rapid fabrication of living vascular tissues by direct culturing of collagen, smooth muscle cells and endothelial cells. In addition, a new protocol for the mechanical characterization of engineered vascular tissues is described.

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Neuroscience

Electrophysiology on Isolated Brainstem-spinal Cord Preparations from Newborn Rodents Allows Neural Respiratory Network Output Recording
Jean-Philippe Rousseau 1, Céline Caravagna 1
1Department of Pediatrics, Laval University

The central respiratory drive is located in the brainstem. Spontaneous respiratory motor output from an isolated brainstem-spinal cord is recorded by placing an electrode on the fourth ventral root. This experimental approach is valuable for pharmacological investigations or the assessment of respiratory challenges and genetic manipulations on rhythmic motor behavior.

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Medicine

Three-Dimensional Culture Assay to Explore Cancer Cell Invasiveness and Satellite Tumor Formation
Marie-France Côté 1, Audrey Turcotte 1,2, Charles Doillon 1,3, Stephane Gobeil 1,2
1CHU de Québec Research Centre, 2Department of Molecular Medicine, Laval University, 3Department of Surgery, Laval University

Cancer cells are embedded in a collagen gel and then sandwiched in an acellular fibrin gel to generate a 3D culture system in which the invasiveness and formation of satellite tumors may be monitored.

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