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Robert Koch Institute

2 ARTICLES PUBLISHED IN JoVE

Biology

Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy

Felix M. Fuchs¹, Marina Raguse^1,2,3, Marcel Fiebrandt², Kazimierz Madela⁴, Peter Awakowicz², Michael Laue⁴, Katharina Stapelmann³, Ralf Moeller¹

¹Department of Radiation Biology, Institute of Aerospace Medicine, Space Microbiology Research Group, German Aerospace Center (DLR e.V.), ²Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Ruhr-University Bochum, ³Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Biomedical Applications of Plasma Technology, Ruhr-University Bochum, ⁴Advanced Light and Electron Microscopy (ZBS 4), Robert Koch Institute

This protocol illustrates the important consecutive steps required to assess the relevance of monitoring vitality parameter and DNA repair processes in reviving Bacillus subtilis spores after treatment with low pressure plasma by tracking fluorescence-labelled DNA repair proteins via time-resolved confocal microscopy and scanning electron microscopy.

Immunology and Infection

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Katherine Schultheis¹, Hubert Schaefer², Holly M. Pugh¹, Bryan S. Yung¹, Janet Oh¹, Jacklyn Nguyen¹, Laurent Humeau¹, Kate E. Broderick¹, Trevor R.F. Smith¹

¹Inovio Pharmaceuticals, Inc, ²Mycotic and Parasitic Agents and Mycobacteria, Robert Koch Institute

The development of the interferon-gamma ELISpot assay for guinea pig PBMCs allows the characterization of T-cell responses in this highly relevant model for studying infectious diseases. We have applied the assay to measure T cell responses associated with intradermal delivery of DNA vaccines.