Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow CytometryDiamantis G. Konstantinidis 1, Suvarnamala Pushkaran 1, Katie Giger 1, Stefanos Manganaris 2, Yi Zheng 1, Theodosia A. Kalfa 1
1Cancer and Blood Diseases Institute, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, 2IBM
The present protocol describes a novel method of identifying a population of enucleating orthochromatic erythroblasts by multi-spectral imaging flow cytometry, providing a visualization of the erythroblast enucleation process.