Modified Tail Vein and Penile Vein Puncture for Blood Sampling in the Rat ModelLaura Charlès 1,2,3, Thomas Agius *4,5, Irina Filz von Reiterdank *1,2,3,6, Janna Hagedorn 1,2,3, Yanis Berkane 1,2,3,7, Hyshem H. Lancia 1,2,3, Basak E. Uygun 2,3,8, Korkut Uygun 2,3,8, Curtis L. Cetrulo Jr. 1,2,3,9, Mark A. Randolph 1,2,3,9, Alexandre G. Lellouch 1,2,3,8
1Vascularized Composite Allotransplantation Laboratory, Massachusetts General Hospital, 2Harvard Medical School, 3Shriners Children’s Boston, 4Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, 5Department of Vascular Surgery, Centre Hospitalier Universitaire Vaudois and University of Lausanne, 6Department of Plastic, Reconstructive and Hand Surgery, University Medical Center Utrecht, 7Department of Plastic, Reconstructive and Aesthetic Surgery, Rennes University Hospital Center (CHU de Rennes), Rennes 1 University, 8Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, 9Plastic Surgery Research Laboratory, Massachusetts General Hospital
Here, we present a protocol to offer rapid, easy, and reliable blood collection alternatives for the rat model. We describe three different blood sampling methods according to the context: tail vein puncture under anesthesia or on a conscious animal, and dorsal penile vein puncture under anesthesia.
