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Veterans Administration TVHS

2 ARTICLES PUBLISHED IN JoVE

Biology

Large Scale Zebrafish-Based In vivo Small Molecule Screen

Jijun Hao¹, Charles H. Williams¹, Morgan E. Webb¹, Charles C. Hong^1,2,3,4

¹Division of Cardiovascular Medicine, Department of Medicine, Vanderbilt University School of Medicine, ²Department of Pharmacology, Vanderbilt University School of Medicine, ³Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, ⁴Research Medicine, Veterans Affairs TVHS, Vanderbilt University School of Medicine

Zebrafish has emerged as a powerful in vivo platform for phenotype-based drug screens and chemical genetic analysis. Here, we demonstrate a simple, practical method for large-scale screening of small molecules using zebrafish embryos.

Biology

Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency

Ada Ao¹, Charles H. Williams¹, Jijun Hao¹, Charles C. Hong^1,2,3,4

We describe the use of a mouse ES cell based assay to identify critical time windows for Wnt/β-catenin and BMP signal activation during cardiogenic induction. The method provides a standardized platform that reliably quantifies cardiogenic efficiency, and it is applicable to the study of other cell lineages.