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Vu Medical Center

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples

Simone Punt¹, Robert J. Baatenburg de Jong², Ekaterina S. Jordanova^1,3

¹Department of Pathology, Leiden University Medical Center, ²Department of Otorhinolaryngology and Head and Neck Surgery, Erasmus University Medical Center, ³Center for Gynecological Oncology Amsterdam, VU Medical Center

Multiparameter fluorescence immunohistochemistry can be used to assess the number, relative distribution, and localization of immune cell populations in the tumor microenvironment. This manuscript describes the use of this technique to analyze T-cell subpopulations in oropharyngeal cancer.

Cancer Research

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Tonny Lagerweij^*1, Maria Pérez-Lanzón^*2, S. Rubina Baglio²

¹Neuro-oncology Research Group, VU Medical Center, ²Exosomes Research Group, Department of Pathology, VU Medical Center

Direct injection of cancer-derived extracellular vesicles (EVs) leads to reprogramming of bone marrow supporting tumor progression; however, which cells mediate this effect is unclear. Herein, we describe a step-by-step protocol to investigate EV-mediated tumor-mesenchymal stem cell (MSC) interactions in vivo, revealing a crucial role for EV-educated MSCs in metastasis.