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University of California - Davis

3 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

Andrew J. McElrone^1,2, Brendan Choat³, Dilworth Y. Parkinson⁴, Alastair A. MacDowell⁴, Craig R. Brodersen⁵

¹U.S. Department of Agriculture, ²Department of Viticulture and Enology, University of California - Davis, ³Hawkesbury Institute for the Environment, University of Western Sydney, ⁴Advanced Light Source, Lawrence Berkeley National Lab, ⁵Citrus Research & Education Center, University of Florida

High resolution x-ray computed tomography (HRCT) is a non-destructive diagnostic imaging technique that can be used to study the structure and function of plant vasculature in 3D. We demonstrate how HRCT facilitates exploration of xylem networks across a wide range of plant tissues and species.

JoVE Journal

Surface Renewal: An Advanced Micrometeorological Method for Measuring and Processing Field-Scale Energy Flux Density Data

Andrew J. McElrone^1,2, Thomas M. Shapland², Arturo Calderon^2,3, Li Fitzmaurice^4,5, Kyaw Tha Paw U⁴, Richard L. Snyder⁴

¹Crops Pathology and Genetics Research Unit, United States Department of Agriculture-Agricultural Research Service, ²Department of Viticulture & Enology, University of California, Davis, ³Faculty of Agricultural Sciences, University of Chile, ⁴Atmospheric Science, University of California, Davis, ⁵URS Corporation Australia Pty. Ltd.

Surface renewal is a micrometeorological method that is being used increasingly to determine energy fluxes, but its technical complexity makes it inaccessible to a broad audience. We describe the steps needed to set up and calibrate a surface renewal field station, to acquire and process data, and to correctly interpret results.

Biochemistry

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

Anthony Asher¹, Iniyan Ganesan¹, Laura Klasek¹, Steven M. Theg¹

¹Department of Plant Biology, University of California - Davis

We present protocols herein for high-yield isolation of physiologically active thylakoids and protein transport assays for the chloroplast twin arginine translocation (cpTat), secretory (cpSec1), and signal recognition particle (cpSRP) pathways.