Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection TomographyAlexander D. Leeper 1, Joanne Farrell 2, J. Michael Dixon 1, Sarah E. Wedden 2, David J. Harrison 1, Elad Katz 1
1Breakthrough Breast Cancer Research Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, 2MRC Technology
We have developed a collagen-based in vitro assay which promotes proliferation and invasion from samples of all breast cancer subtypes. Optical Projection Tomography, a three dimensional microscopy technique was utilised to visualise and quantify tumour expansion. This assay may be used to quantify drug response of individual tumour samples.
