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University of Rochester Medical Center

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

Oksana Polesskaya¹, Anita Sun², Gheorghe Salahura², Jharon N. Silva¹, Stephen Dewhurst¹, Karl Kasischke³

¹Department of Microbiology and Immunology, University of Rochester Medical Center, ²Center for Neural Development and Disease, University of Rochester Medical Center, ³Deptartment of Neurology, Center for Neural Development and Disease, University of Rochester Medical Center

Here we describe a method to directly visualize microregional tissue hypoxia in the mouse cortex in vivo. It is based on concurrent two-photon imaging of nicotinamide adenine dinucleotide (NADH) and the cortical microcirculation. This method is useful for high resolution analysis of tissue oxygen supply.

Neuroscience

Assessing Neurodegenerative Phenotypes in Drosophila Dopaminergic Neurons by Climbing Assays and Whole Brain Immunostaining

Maria Cecilia Barone¹, Dirk Bohmann¹

¹Department of Biomolecular Genetics, University of Rochester Medical Center

Here we describe two assays that have been established to study age-dependent neurodegeneration of dopaminergic (DA) neurons in Drosophila: the climbing/startle-induced negative geotaxis assay which allows to study the functional effects of DA neurons degeneration and the tyrosine hydroxylase immunostaining which is used to identify and count DA neurons in whole brain mounts.